Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2343126..2343751 Replicon chromosome
Accession NZ_CP072555
Organism Klebsiella pneumoniae strain KP2648

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J7339_RS11535 Protein ID WP_002882817.1
Coordinates 2343126..2343509 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J7339_RS11540 Protein ID WP_004150355.1
Coordinates 2343509..2343751 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7339_RS11520 (J7339_11470) 2340492..2341394 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J7339_RS11525 (J7339_11475) 2341391..2342026 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J7339_RS11530 (J7339_11480) 2342023..2342952 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J7339_RS11535 (J7339_11485) 2343126..2343509 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J7339_RS11540 (J7339_11490) 2343509..2343751 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J7339_RS11545 (J7339_11495) 2343956..2344873 + 918 WP_002882812.1 alpha/beta hydrolase -
J7339_RS11550 (J7339_11500) 2344887..2345828 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J7339_RS11555 (J7339_11505) 2345873..2346310 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J7339_RS11560 (J7339_11510) 2346307..2347167 - 861 WP_002882807.1 virulence factor BrkB family protein -
J7339_RS11565 (J7339_11515) 2347161..2347760 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T198022 WP_002882817.1 NZ_CP072555:c2343509-2343126 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T198022 NZ_CP096839:3728790-3729047 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT198022 WP_004150355.1 NZ_CP072555:c2343751-2343509 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT198022 NZ_CP096839:3728518-3728793 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References