Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 242616..243204 | Replicon | plasmid pH7-250 |
| Accession | NZ_CP072550 | ||
| Organism | Acinetobacter lwoffii strain H7 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | J7649_RS15445 | Protein ID | WP_000438825.1 |
| Coordinates | 242917..243204 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | J7649_RS15440 | Protein ID | WP_004728120.1 |
| Coordinates | 242616..242930 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7649_RS15410 (J7649_15410) | 237775..239100 | - | 1326 | WP_001280601.1 | ISNCY family transposase | - |
| J7649_RS15415 (J7649_15415) | 239278..239697 | + | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| J7649_RS15420 (J7649_15420) | 239777..240058 | - | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| J7649_RS15425 (J7649_15425) | 240059..240361 | - | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| J7649_RS15430 (J7649_15430) | 240481..241806 | - | 1326 | WP_001280601.1 | ISNCY family transposase | - |
| J7649_RS15435 (J7649_15435) | 241997..242527 | + | 531 | WP_000172759.1 | hypothetical protein | - |
| J7649_RS15440 (J7649_15440) | 242616..242930 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| J7649_RS15445 (J7649_15445) | 242917..243204 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| J7649_RS15450 (J7649_15450) | 243451..243783 | - | 333 | WP_117008139.1 | MerR family DNA-binding protein | - |
| J7649_RS15455 (J7649_15455) | 243809..244192 | + | 384 | WP_001055589.1 | transposase | - |
| J7649_RS15460 (J7649_15460) | 244189..244524 | + | 336 | WP_000618091.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| J7649_RS15465 (J7649_15465) | 244668..246167 | + | 1500 | Protein_232 | IS66-like element ISAba16 family transposase | - |
| J7649_RS16920 (J7649_15470) | 246198..246440 | + | 243 | Protein_233 | integrase core domain-containing protein | - |
| J7649_RS16830 | 246450..247043 | - | 594 | WP_004758987.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aph(3')-VIa / sul1 / blaPER-1 / qacE / ARR-3 / floR / mph(E) / msr(E) | - | 1..250175 | 250175 | |
| - | inside | IScluster/Tn | floR / mph(E) / msr(E) | - | 229697..246245 | 16548 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T198014 WP_000438825.1 NZ_CP072550:c243204-242917 [Acinetobacter lwoffii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T198014 NZ_CP096839:c3033504-3033109 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT198014 WP_004728120.1 NZ_CP072550:c242930-242616 [Acinetobacter lwoffii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT198014 NZ_CP096839:c3033083-3032817 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |