Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PumA-relB/upstrm_HI1419-dnstrm_HI1420 |
| Location | 239777..240361 | Replicon | plasmid pH7-250 |
| Accession | NZ_CP072550 | ||
| Organism | Acinetobacter lwoffii strain H7 | ||
Toxin (Protein)
| Gene name | PumA | Uniprot ID | N8Q7V3 |
| Locus tag | J7649_RS15425 | Protein ID | WP_000286964.1 |
| Coordinates | 240059..240361 (-) | Length | 101 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | J7649_RS15420 | Protein ID | WP_000985609.1 |
| Coordinates | 239777..240058 (-) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7649_RS15395 (J7649_15395) | 235098..236573 | - | 1476 | WP_000052512.1 | ABC-F type ribosomal protection protein Msr(E) | - |
| J7649_RS15400 (J7649_15400) | 237066..237338 | - | 273 | WP_000369781.1 | NadS family protein | - |
| J7649_RS15405 (J7649_15405) | 237331..237651 | - | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| J7649_RS15410 (J7649_15410) | 237775..239100 | - | 1326 | WP_001280601.1 | ISNCY family transposase | - |
| J7649_RS15415 (J7649_15415) | 239278..239697 | + | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| J7649_RS15420 (J7649_15420) | 239777..240058 | - | 282 | WP_000985609.1 | putative addiction module antidote protein | Antitoxin |
| J7649_RS15425 (J7649_15425) | 240059..240361 | - | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| J7649_RS15430 (J7649_15430) | 240481..241806 | - | 1326 | WP_001280601.1 | ISNCY family transposase | - |
| J7649_RS15435 (J7649_15435) | 241997..242527 | + | 531 | WP_000172759.1 | hypothetical protein | - |
| J7649_RS15440 (J7649_15440) | 242616..242930 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | - |
| J7649_RS15445 (J7649_15445) | 242917..243204 | - | 288 | WP_000438825.1 | BrnT family toxin | - |
| J7649_RS15450 (J7649_15450) | 243451..243783 | - | 333 | WP_117008139.1 | MerR family DNA-binding protein | - |
| J7649_RS15455 (J7649_15455) | 243809..244192 | + | 384 | WP_001055589.1 | transposase | - |
| J7649_RS15460 (J7649_15460) | 244189..244524 | + | 336 | WP_000618091.1 | IS66 family insertion sequence element accessory protein TnpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aph(3')-VIa / sul1 / blaPER-1 / qacE / ARR-3 / floR / mph(E) / msr(E) | - | 1..250175 | 250175 | |
| - | inside | IScluster/Tn | floR / mph(E) / msr(E) | - | 229697..246245 | 16548 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 101 a.a. Molecular weight: 11573.07 Da Isoelectric Point: 4.6838
>T198013 WP_000286964.1 NZ_CP072550:c240361-240059 [Acinetobacter lwoffii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL
Download Length: 303 bp
>T198013 NZ_CP096839:c3032820-3032404 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10109.67 Da Isoelectric Point: 8.0018
>AT198013 WP_000985609.1 NZ_CP072550:c240058-239777 [Acinetobacter lwoffii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI
Download Length: 282 bp
>AT198013 NZ_CP096839:c3033083-3032817 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|