Detailed information of TA system TA198007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 99209..99879
• Replicon: chromosome
• Accession: NZ_CP072546
• Organism: Brucella sp. 458

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J8E27_RS00515
• Protein ID: WP_076770566.1
• Coordinates: 99475..99879 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D1CXH4
• Locus tag: J8E27_RS00510
• Protein ID: WP_008504606.1
• Coordinates: 99209..99478 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8E27_RS00490 (J8E27_00490)	96227..96424	+	198	WP_002963959.1	DUF3008 family protein	-
J8E27_RS00495 (J8E27_00495)	96469..96882	-	414	WP_008935646.1	hypothetical protein	-
J8E27_RS00500 (J8E27_00500)	97154..98125	-	972	WP_209408617.1	extensin family protein	-
J8E27_RS00505 (J8E27_00505)	98460..99077	+	618	WP_076779584.1	30S ribosomal protein S4	-
J8E27_RS00510 (J8E27_00510)	99209..99478	+	270	WP_008504606.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J8E27_RS00515 (J8E27_00515)	99475..99879	+	405	WP_076770566.1	type II toxin-antitoxin system VapC family toxin	Toxin
J8E27_RS00520 (J8E27_00520)	100176..101057	+	882	WP_180881897.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
J8E27_RS00525 (J8E27_00525)	101044..101871	+	828	WP_209409364.1	inositol monophosphatase family protein	-
J8E27_RS00530 (J8E27_00530)	101925..103190	-	1266	WP_209408618.1	multidrug effflux MFS transporter	-
J8E27_RS00535 (J8E27_00535)	103461..103793	-	333	WP_008505520.1	Grx4 family monothiol glutaredoxin	-
J8E27_RS00540 (J8E27_00540)	103978..104211	-	234	WP_002963968.1	BolA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14305.62 Da
• Isoelectric Point: 5.6357

>T198007 WP_076770566.1 NZ_CP072546:99475-99879 [Brucella sp. 458]
MSKLYMLDTNIVSDLARNPHGPVAKHIARVGADAICVSIITACELRYGCAKKGSPRLLAQIEAILDSIEILPLDVPADTE
YGGIRAELEADGKPIGPNDFLIAAHACALGAVLVTANVGEFTRIRKLAVENWLN

Nucleotide

• Length: 405 bp

>T198007 NZ_CP096839:2781429-2781854 [Mycobacterium tuberculosis variant bovis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10046.62 Da
• Isoelectric Point: 7.2059

>AT198007 WP_008504606.1 NZ_CP072546:99209-99478 [Brucella sp. 458]
MAHARRIEPTPAREAKLFRNNKSQAVRIPADFEFPGNRVLIHREGDKLIIEPVRRKNLLEVLAGLKPLGPEDAFPDIDDT
ALPLKDVDL

Nucleotide

• Length: 270 bp

>AT198007 NZ_CP096839:2781202-2781423 [Mycobacterium tuberculosis variant bovis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7L7MKG1