Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 72784..73385 Replicon plasmid pAW1.7_1
Accession NZ_CP072540
Organism Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag J7886_RS24320 Protein ID WP_001216034.1
Coordinates 73005..73385 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag J7886_RS24315 Protein ID WP_001190712.1
Coordinates 72784..73005 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7886_RS24305 (J7886_24300) 69792..71048 - 1257 WP_054626026.1 restriction endonuclease subunit S -
J7886_RS24310 (J7886_24305) 71045..72601 - 1557 WP_060615238.1 type I restriction-modification system subunit M -
J7886_RS24315 (J7886_24310) 72784..73005 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J7886_RS24320 (J7886_24315) 73005..73385 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J7886_RS24325 (J7886_24320) 73390..73569 + 180 WP_009446826.1 hypothetical protein -
J7886_RS24330 (J7886_24325) 73597..74640 + 1044 WP_023356283.1 DUF968 domain-containing protein -
J7886_RS24335 (J7886_24330) 74729..75181 + 453 WP_001326849.1 late promoter-activating protein -
J7886_RS24340 (J7886_24335) 75267..76460 + 1194 WP_054625992.1 terminase -
J7886_RS24345 (J7886_24340) 76460..77728 + 1269 WP_251364675.1 terminase -
J7886_RS25310 77782..77943 + 162 WP_251364676.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..103129 103129


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T198005 WP_001216034.1 NZ_CP072540:73005-73385 [Escherichia coli O128ac:H12]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T198005 NZ_CP096839:c2350282-2349848 [Mycobacterium tuberculosis variant bovis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT198005 WP_001190712.1 NZ_CP072540:72784-73005 [Escherichia coli O128ac:H12]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT198005 NZ_CP096839:c2350543-2350289 [Mycobacterium tuberculosis variant bovis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References