Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4493942..4494439 Replicon chromosome
Accession NZ_CP072539
Organism Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J7886_RS21735 Protein ID WP_001173343.1
Coordinates 4493942..4494115 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag J7886_RS21740 Protein ID WP_001425728.1
Coordinates 4494143..4494439 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7886_RS21725 (4490612) 4490612..4492069 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
J7886_RS21730 (4492306) 4492306..4493823 + 1518 WP_001295074.1 lysine--tRNA ligase -
J7886_RS21735 (4493942) 4493942..4494115 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J7886_RS21740 (4494143) 4494143..4494439 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J7886_RS21745 (4494666) 4494666..4494938 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J7886_RS21750 (4494950) 4494950..4495180 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
J7886_RS21755 (4495361) 4495361..4496992 + 1632 WP_001216486.1 sensor histidine kinase -
J7886_RS21760 (4496989) 4496989..4497708 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4463152..4494439 31287


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T198004 WP_001173343.1 NZ_CP072539:c4494115-4493942 [Escherichia coli O128ac:H12]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T198004 NZ_CP096839:2308892-2309302 [Mycobacterium tuberculosis variant bovis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT198004 WP_001425728.1 NZ_CP072539:c4494439-4494143 [Escherichia coli O128ac:H12]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT198004 NZ_CP096839:2308666-2308899 [Mycobacterium tuberculosis variant bovis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References