Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4287371..4287947 Replicon chromosome
Accession NZ_CP072539
Organism Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag J7886_RS20735 Protein ID WP_001297643.1
Coordinates 4287660..4287947 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag J7886_RS20730 Protein ID WP_000063160.1
Coordinates 4287371..4287673 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7886_RS20715 (4283967) 4283967..4286117 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
J7886_RS20720 (4286167) 4286167..4286370 + 204 WP_000467859.1 YbdD/YjiX family protein -
J7886_RS20725 (4286381) 4286381..4287337 + 957 WP_001297640.1 GTPase -
J7886_RS20730 (4287371) 4287371..4287673 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
J7886_RS20735 (4287660) 4287660..4287947 - 288 WP_001297643.1 BrnT family toxin Toxin
J7886_RS20740 (4288146) 4288146..4288842 - 697 Protein_4089 IS1 family transposase -
J7886_RS20745 (4289157) 4289157..4290389 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
J7886_RS20750 (4290430) 4290430..4291710 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4288146..4288355 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T198002 WP_001297643.1 NZ_CP072539:c4287947-4287660 [Escherichia coli O128ac:H12]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T198002 NZ_CP096839:c2217484-2217140 [Mycobacterium tuberculosis variant bovis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT198002 WP_000063160.1 NZ_CP072539:c4287673-4287371 [Escherichia coli O128ac:H12]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT198002 NZ_CP096839:c2217726-2217478 [Mycobacterium tuberculosis variant bovis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References