Detailed information of TA system TA197999

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3921280..3921959
• Replicon: chromosome
• Accession: NZ_CP072539
• Organism: Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2I8S2T2
• Locus tag: J7886_RS18955
• Protein ID: WP_008324514.1
• Coordinates: 3921618..3921959 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A2I8S2U6
• Locus tag: J7886_RS18950
• Protein ID: WP_008324516.1
• Coordinates: 3921280..3921597 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7886_RS18905 (3916721)	3916721..3917542	+	822	WP_054626066.1	DUF932 domain-containing protein	-
J7886_RS18910 (3917760)	3917760..3918461	+	702	WP_054626065.1	WYL domain-containing protein	-
J7886_RS18915 (3918498)	3918498..3918734	+	237	WP_001115854.1	protein YpjK	-
J7886_RS18920 (3918734)	3918734..3919177	+	444	WP_008324520.1	lipoprotein YafY	-
J7886_RS18925 (3919200)	3919200..3919667	+	468	WP_001547765.1	protein YkfB	-
J7886_RS18930 (3919744)	3919744..3919983	+	240	WP_000194654.1	DUF905 family protein	-
J7886_RS18935 (3920081)	3920081..3920539	+	459	WP_000211838.1	antirestriction protein	-
J7886_RS18940 (3920555)	3920555..3921031	+	477	WP_000811693.1	RadC family protein	-
J7886_RS18945 (3921040)	3921040..3921267	+	228	WP_008324517.1	DUF987 domain-containing protein	-
J7886_RS18950 (3921280)	3921280..3921597	+	318	WP_008324516.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J7886_RS18955 (3921618)	3921618..3921959	+	342	WP_008324514.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J7886_RS18965 (3922531)	3922531..3923784	-	1254	WP_000893260.1	glutamate-5-semialdehyde dehydrogenase	-
J7886_RS18970 (3923796)	3923796..3924899	-	1104	WP_001285288.1	glutamate 5-kinase	-
J7886_RS18975 (3925187)	3925187..3926242	+	1056	WP_000749881.1	phosphoporin PhoE	-
J7886_RS18980 (3926281)	3926281..3926682	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3913073..3922179	9106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12919.98 Da
• Isoelectric Point: 9.6543

>T197999 WP_008324514.1 NZ_CP072539:3921618-3921959 [Escherichia coli O128ac:H12]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T197999 NZ_CP096839:c2199530-2199234 [Mycobacterium tuberculosis variant bovis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11787.33 Da
• Isoelectric Point: 7.3476

>AT197999 WP_008324516.1 NZ_CP072539:3921280-3921597 [Escherichia coli O128ac:H12]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT197999 NZ_CP096839:c2199778-2199527 [Mycobacterium tuberculosis variant bovis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S2T2

Antitoxin

• AlphaFold DB: A0A2I8S2U6