Detailed information of TA system TA197997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2687118..2687756
• Replicon: chromosome
• Accession: NZ_CP072539
• Organism: Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J7886_RS13060
• Protein ID: WP_000813794.1
• Coordinates: 2687580..2687756 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J7886_RS13055
• Protein ID: WP_001270286.1
• Coordinates: 2687118..2687534 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7886_RS13035 (2682270)	2682270..2683211	-	942	WP_001251313.1	ABC transporter permease	-
J7886_RS13040 (2683212)	2683212..2684225	-	1014	WP_000220434.1	ABC transporter ATP-binding protein	-
J7886_RS13045 (2684243)	2684243..2685388	-	1146	WP_000047430.1	ABC transporter substrate-binding protein	-
J7886_RS13050 (2685633)	2685633..2687039	-	1407	WP_000760630.1	PLP-dependent aminotransferase family protein	-
J7886_RS13055 (2687118)	2687118..2687534	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J7886_RS13060 (2687580)	2687580..2687756	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J7886_RS13065 (2687978)	2687978..2688208	+	231	WP_072188679.1	YncJ family protein	-
J7886_RS13070 (2688300)	2688300..2690261	-	1962	WP_001488038.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J7886_RS13075 (2690334)	2690334..2690870	-	537	WP_000429169.1	DNA-binding transcriptional regulator SutR	-
J7886_RS13080 (2690923)	2690923..2692137	+	1215	WP_001324881.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T197997 WP_000813794.1 NZ_CP072539:c2687756-2687580 [Escherichia coli O128ac:H12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T197997 NZ_CP096839:c2190526-2190197 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT197997 WP_001270286.1 NZ_CP072539:c2687534-2687118 [Escherichia coli O128ac:H12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT197997 NZ_CP096839:c2190900-2190523 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• PDB: 6HPB