Detailed information of TA system TA197996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2590661..2592249
• Replicon: chromosome
• Accession: NZ_CP072539
• Organism: Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J7886_RS12650
• Protein ID: WP_054626327.1
• Coordinates: 2590927..2592249 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J7886_RS12645
• Protein ID: WP_001301023.1
• Coordinates: 2590661..2590927 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7886_RS12645 (2590661)	2590661..2590927	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J7886_RS12650 (2590927)	2590927..2592249	+	1323	WP_054626327.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J7886_RS12655 (2592949)	2592949..2594111	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
J7886_RS12660 (2594125)	2594125..2594607	-	483	WP_225858593.1	hypothetical protein	-
J7886_RS12665 (2594625)	2594625..2595065	-	441	WP_016239970.1	hypothetical protein	-
J7886_RS12670 (2595218)	2595218..2596579	-	1362	WP_016239969.1	FRG domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49290.79 Da
• Isoelectric Point: 8.2482

>T197996 WP_054626327.1 NZ_CP072539:2590927-2592249 [Escherichia coli O128ac:H12]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVQRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T197996 NZ_CP096839:1955225-1955473 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT197996 WP_001301023.1 NZ_CP072539:2590661-2590927 [Escherichia coli O128ac:H12]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT197996 NZ_CP096839:1955013-1955225 [Mycobacterium tuberculosis variant bovis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA