Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 445424..446055 Replicon chromosome
Accession NZ_CP072539
Organism Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag J7886_RS01885 Protein ID WP_001259385.1
Coordinates 445424..445699 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J7886_RS01890 Protein ID WP_000593555.1
Coordinates 445696..446055 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7886_RS01870 (440428) 440428..441495 + 1068 WP_000361470.1 HlyD family secretion protein -
J7886_RS01875 (441492) 441492..444227 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
J7886_RS01880 (444227) 444227..445351 + 1125 WP_001488398.1 ABC transporter permease -
J7886_RS01885 (445424) 445424..445699 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
J7886_RS01890 (445696) 445696..446055 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J7886_RS01895 (446175) 446175..446576 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J7886_RS01900 (446582) 446582..447388 - 807 WP_054626010.1 nickel import ATP-binding protein NikE -
J7886_RS01905 (447385) 447385..448149 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J7886_RS01910 (448149) 448149..448982 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J7886_RS01915 (448979) 448979..449923 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 374705..447388 72683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T197978 WP_001259385.1 NZ_CP072539:445424-445699 [Escherichia coli O128ac:H12]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T197978 NZ_CP096839:714575-714970 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT197978 WP_000593555.1 NZ_CP072539:445696-446055 [Escherichia coli O128ac:H12]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT197978 NZ_CP096839:714321-714575 [Mycobacterium tuberculosis variant bovis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References