Detailed information of TA system TA197977

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 145236..145915
• Replicon: chromosome
• Accession: NZ_CP072539
• Organism: Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: J7886_RS00605
• Protein ID: WP_032170241.1
• Coordinates: 145236..145577 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: J7886_RS00610
• Protein ID: WP_054626043.1
• Coordinates: 145598..145915 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7886_RS00580 (140735)	140735..141658	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
J7886_RS00585 (141769)	141769..142953	-	1185	WP_001172876.1	sugar efflux transporter	-
J7886_RS00590 (143389)	143389..143511	-	123	Protein_118	RhuM family protein	-
J7886_RS00595 (143786)	143786..144169	-	384	WP_032170243.1	hypothetical protein	-
J7886_RS25095 (144183)	144183..144371	-	189	WP_251364672.1	hypothetical protein	-
J7886_RS25100 (144506)	144506..144943	+	438	WP_251364673.1	toll/interleukin-1 receptor domain-containing protein	-
J7886_RS00605 (145236)	145236..145577	-	342	WP_032170241.1	TA system toxin CbtA family protein	Toxin
J7886_RS00610 (145598)	145598..145915	-	318	WP_054626043.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J7886_RS00615 (145934)	145934..146155	-	222	WP_000691995.1	DUF987 domain-containing protein	-
J7886_RS00620 (146164)	146164..146640	-	477	WP_001618788.1	RadC family protein	-
J7886_RS00625 (146656)	146656..147114	-	459	WP_000211838.1	antirestriction protein	-
J7886_RS00630 (147212)	147212..147451	-	240	WP_032170520.1	DUF905 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	144242..167646	23404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12821.81 Da
• Isoelectric Point: 10.0426

>T197977 WP_032170241.1 NZ_CP072539:c145577-145236 [Escherichia coli O128ac:H12]
MKILPATTSRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIQNHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNTAVR

Nucleotide

• Length: 342 bp

>T197977 NZ_CP096839:708916-709317 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12029.65 Da
• Isoelectric Point: 7.0262

>AT197977 WP_054626043.1 NZ_CP072539:c145915-145598 [Escherichia coli O128ac:H12]
MSNITWGLQRNITPCLGARLVQEGNRLHYLADRTSITGKFSDIECRKLDETFPHFIRQMESMLTTGEMNPHHAHYVILYH
NGFTCEADTLGSCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT197977 NZ_CP096839:708692-708919 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA