Detailed information of TA system TA197976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 91205..91790
• Replicon: plasmid pDETAB5
• Accession: NZ_CP072528
• Organism: Acinetobacter baumannii strain DETAB-E227

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N9M3U8
• Locus tag: J7D37_RS18920
• Protein ID: WP_005155676.1
• Coordinates: 91503..91790 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9LW73
• Locus tag: J7D37_RS18915
• Protein ID: WP_005155677.1
• Coordinates: 91205..91516 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7D37_RS18895 (J7D37_18895)	88167..89389	-	1223	Protein_87	IS256 family transposase	-
J7D37_RS18900 (J7D37_18900)	89483..89965	-	483	Protein_88	zinc-binding dehydrogenase	-
J7D37_RS18905 (J7D37_18905)	90028..90732	+	705	WP_001067783.1	IS6-like element IS1008 family transposase	-
J7D37_RS18910 (J7D37_18910)	90787..91126	+	340	Protein_90	IS4 family transposase	-
J7D37_RS18915 (J7D37_18915)	91205..91516	-	312	WP_005155677.1	BrnA antitoxin family protein	Antitoxin
J7D37_RS18920 (J7D37_18920)	91503..91790	-	288	WP_005155676.1	BrnT family toxin	Toxin
J7D37_RS18925 (J7D37_18925)	92093..93126	+	1034	Protein_93	ISNCY family transposase	-
J7D37_RS18930 (J7D37_18930)	93132..93323	-	192	Protein_94	IS4 family transposase	-
J7D37_RS18935 (J7D37_18935)	93352..94052	-	701	Protein_95	IS1 family transposase	-
J7D37_RS18940 (J7D37_18940)	94113..94349	-	237	Protein_96	IS4 family transposase	-
J7D37_RS18945 (J7D37_18945)	94476..95375	-	900	WP_005166156.1	ParB/RepB/Spo0J family partition protein	-
J7D37_RS18950 (J7D37_18950)	95394..96170	-	777	WP_005166158.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / sul2 / msr(E) / mph(E) / blaNDM-1	-	1..97035	97035
	-	inside	IScluster/Tn	blaNDM-1	-	79724..94352	14628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11275.66 Da
• Isoelectric Point: 5.6113

>T197976 WP_005155676.1 NZ_CP072528:c91790-91503 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T197976 NZ_CP096839:701396-701797 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11984.58 Da
• Isoelectric Point: 6.7277

>AT197976 WP_005155677.1 NZ_CP072528:c91516-91205 [Acinetobacter baumannii]
MSMVRYTREELNENFSNKQDAEVEHLLAKGTVPDDQLDLSDIPEITDWSNAIRHGQFFRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT197976 NZ_CP096839:701154-701399 [Mycobacterium tuberculosis variant bovis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: N9M3U8

Antitoxin

• AlphaFold DB: N9LW73