Detailed information of TA system TA197967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1327803..1328719
• Replicon: chromosome
• Accession: NZ_CP072525
• Organism: Bacillus subtilis strain YB-04

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: J7W01_RS06920
• Protein ID: WP_003244695.1
• Coordinates: 1327973..1328719 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: J7W01_RS06915
• Protein ID: WP_003232646.1
• Coordinates: 1327803..1327973 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7W01_RS06880 (1324666)	1324666..1324995	+	330	WP_041850928.1	XkdW family protein	-
J7W01_RS06885 (1324992)	1324992..1325156	+	165	WP_041850927.1	XkdX family protein	-
J7W01_RS06890 (1325200)	1325200..1326039	+	840	WP_041850926.1	phage-like element PBSX protein XepA	-
J7W01_RS06895 (1326092)	1326092..1326361	+	270	WP_003232655.1	hemolysin XhlA family protein	-
J7W01_RS06900 (1326374)	1326374..1326637	+	264	WP_003232653.1	phage holin	-
J7W01_RS06905 (1326650)	1326650..1327543	+	894	WP_041850925.1	N-acetylmuramoyl-L-alanine amidase	-
J7W01_RS06910 (1327580)	1327580..1327717	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
J7W01_RS06915 (1327803)	1327803..1327973	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
J7W01_RS06920 (1327973)	1327973..1328719	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
J7W01_RS06925 (1328829)	1328829..1329830	-	1002	WP_003232642.1	inorganic phosphate transporter	-
J7W01_RS06930 (1329843)	1329843..1330460	-	618	WP_003218470.1	DUF47 domain-containing protein	-
J7W01_RS06935 (1330736)	1330736..1332052	-	1317	WP_041850924.1	serine/threonine exchanger	-
J7W01_RS06940 (1332440)	1332440..1333390	+	951	WP_041344800.1	ring-cleaving dioxygenase	-
J7W01_RS06945 (1333491)	1333491..1333637	+	147	WP_120363335.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T197967 WP_003244695.1 NZ_CP072525:c1328719-1327973 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T197967 NZ_CP096838:49827-50108 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT197967 WP_003232646.1 NZ_CP072525:c1327973-1327803 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT197967 NZ_CP096838:49586-49837 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8