Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509931..510567 Replicon chromosome
Accession NZ_CP072525
Organism Bacillus subtilis strain YB-04

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag J7W01_RS02585 Protein ID WP_003156187.1
Coordinates 510217..510567 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag J7W01_RS02580 Protein ID WP_003225183.1
Coordinates 509931..510212 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7W01_RS02560 (506291) 506291..506890 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
J7W01_RS02565 (506985) 506985..507350 + 366 WP_015252768.1 holo-ACP synthase -
J7W01_RS02570 (507516) 507516..508532 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
J7W01_RS02575 (508646) 508646..509815 + 1170 WP_015252766.1 alanine racemase -
J7W01_RS02580 (509931) 509931..510212 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
J7W01_RS02585 (510217) 510217..510567 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J7W01_RS02590 (510682) 510682..511506 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
J7W01_RS02595 (511511) 511511..511876 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
J7W01_RS02600 (511880) 511880..512281 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
J7W01_RS02605 (512293) 512293..513300 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
J7W01_RS02610 (513362) 513362..513691 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
J7W01_RS02615 (513688) 513688..514170 + 483 WP_003234299.1 anti-sigma B factor RsbW -
J7W01_RS02620 (514136) 514136..514924 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
J7W01_RS02625 (514924) 514924..515523 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T197966 WP_003156187.1 NZ_CP072525:510217-510567 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T197966 NZ_CP096837:c4344575-4344402 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT197966 WP_003225183.1 NZ_CP072525:509931-510212 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT197966 NZ_CP096837:c4344899-4344603 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References