Detailed information of TA system TA197963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2256466..2257686
• Replicon: chromosome
• Accession: NZ_CP072521
• Organism: Burkholderia seminalis strain 869T2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8A8D8N5
• Locus tag: DT99_RS26650
• Protein ID: WP_034189314.1
• Coordinates: 2257105..2257686 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DT99_RS26645
• Protein ID: WP_034189315.1
• Coordinates: 2256466..2257023 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DT99_RS26620 (DT99_026620)	2251938..2252669	-	732	WP_034189320.1	ribonuclease T2	-
DT99_RS26625 (DT99_026625)	2252900..2253289	+	390	WP_034189319.1	OsmC family protein	-
DT99_RS26630 (DT99_026630)	2253373..2253951	-	579	WP_034189318.1	hypothetical protein	-
DT99_RS26635 (DT99_026635)	2254046..2255449	-	1404	WP_034189317.1	membrane protein	-
DT99_RS26640 (DT99_026640)	2255790..2256428	+	639	WP_034189316.1	hypothetical protein	-
DT99_RS26645 (DT99_026645)	2256466..2257023	+	558	WP_034189315.1	helix-turn-helix transcriptional regulator	Antitoxin
DT99_RS26650 (DT99_026650)	2257105..2257686	+	582	WP_034189314.1	HD domain-containing protein	Toxin
DT99_RS26655 (DT99_026655)	2257742..2258470	+	729	WP_034189510.1	class I SAM-dependent methyltransferase	-
DT99_RS26660 (DT99_026660)	2258733..2259917	+	1185	WP_034189313.1	macrolide transporter subunit MacA	-
DT99_RS26665 (DT99_026665)	2259920..2261977	+	2058	WP_034189312.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (6-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-62)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21431.08 Da
• Isoelectric Point: 5.1050

>T197963 WP_034189314.1 NZ_CP072521:2257105-2257686 [Burkholderia seminalis]
MNPDTIRARLDFLREAERLKDVLRSGHTSNGRPESTAEHSWRLCLMALVFADALPGIDMLKLLKLCIVHDLGEALHGDIP
AIEQAAHPDKSVHERDDLLTLAGSLDNAQRDAIVALWEEYEAAASPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFSAIREIVDADTQRRIDAGRQHA

Nucleotide

• Length: 582 bp

>T197963 NZ_CP096837:c4011009-4010695 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20300.29 Da
• Isoelectric Point: 11.0752

>AT197963 WP_034189315.1 NZ_CP072521:2256466-2257023 [Burkholderia seminalis]
MTASPLHTPPLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEAGRQQMSPDERARAERLLAARLDSAGDHALARLIAG
SAGRLHLVCDLTHRLLACSPARAAEFSQPLSMLLGTSLWRYATPEIVRMEAALDPLGWHERAGPPSVEFDTGANASRIVP
IRGSRCRWTRMTLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT197963 NZ_CP096837:c4011245-4011012 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A8A8D8N5