Detailed information of TA system TA197962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2084209..2084864
• Replicon: chromosome
• Accession: NZ_CP072521
• Organism: Burkholderia seminalis strain 869T2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8A8D8B6
• Locus tag: DT99_RS25905
• Protein ID: WP_034189437.1
• Coordinates: 2084209..2084628 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DT99_RS25910
• Protein ID: WP_034189436.1
• Coordinates: 2084625..2084864 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DT99_RS25880 (DT99_025880)	2079654..2080448	+	795	WP_034189440.1	AraC family transcriptional regulator	-
DT99_RS25885 (DT99_025885)	2080629..2081582	+	954	WP_034189526.1	DMT family transporter	-
DT99_RS25890 (DT99_025890)	2081769..2082317	+	549	WP_034189439.1	hypothetical protein	-
DT99_RS25895 (DT99_025895)	2082415..2082549	-	135	WP_021158165.1	entericidin A/B family lipoprotein	-
DT99_RS25900 (DT99_025900)	2082782..2084077	+	1296	WP_034189438.1	aspartate carbamoyltransferase	-
DT99_RS25905 (DT99_025905)	2084209..2084628	-	420	WP_034189437.1	type II toxin-antitoxin system VapC family toxin	Toxin
DT99_RS25910 (DT99_025910)	2084625..2084864	-	240	WP_034189436.1	Arc family DNA-binding protein	Antitoxin
DT99_RS25915 (DT99_025915)	2085186..2087429	+	2244	WP_034189435.1	TonB-dependent siderophore receptor	-
DT99_RS25920 (DT99_025920)	2087433..2088188	+	756	WP_034189434.1	tetratricopeptide repeat protein	-
DT99_RS25925 (DT99_025925)	2088185..2088868	+	684	WP_034189433.1	Fe2+-dependent dioxygenase	-
DT99_RS25930 (DT99_025930)	2088989..2089228	+	240	WP_080287878.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DT99_RS25935 (DT99_025935)	2089225..2089620	+	396	WP_034189524.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (6-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14930.18 Da
• Isoelectric Point: 6.4673

>T197962 WP_034189437.1 NZ_CP072521:c2084628-2084209 [Burkholderia seminalis]
MILVDTNVISEPLRREPSAAVVEWLDAQHVETLFLSAVSLAELRFGVAALPEGRGRDWLHQGIEQRVVPLFRGRILPFDD
AASKAYASLCARARAAGNAVAVADGYIAATAEANGLIVAARDVAPFKALGLRVIDPWAR

Nucleotide

• Length: 420 bp

>T197962 NZ_CP096837:3776099-3776338 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATATTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8718.92 Da
• Isoelectric Point: 5.1133

>AT197962 WP_034189436.1 NZ_CP072521:c2084864-2084625 [Burkholderia seminalis]
MPVLTVRNLPDEVHRALRIRAARHGRSTEAEVRDILEQAVLPEGRLKLGTLLAEFGPEAGDDDFGIQRDKAPAAPMSFE

Nucleotide

• Length: 240 bp

>AT197962 NZ_CP096837:3775836-3776096 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A8A8D8B6