197960

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-dnstrm_HI1420
Location 739006..739647 Replicon chromosome
Accession NZ_CP072521
Organism Burkholderia seminalis strain 869T2

Toxin (Protein)


Gene name relE Uniprot ID A0A8A8DBW7
Locus tag DT99_RS20030 Protein ID WP_034185661.1
Coordinates 739309..739647 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag DT99_RS20025 Protein ID WP_034185660.1
Coordinates 739006..739305 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DT99_RS20000 (DT99_020000) 734202..735473 + 1272 WP_034185965.1 anthranilate 1,2-dioxygenase large subunit AndAc -
DT99_RS20005 (DT99_020005) 735488..735964 + 477 WP_006490977.1 anthranilate 1,2-dioxygenase small subunit AndAd -
DT99_RS20010 (DT99_020010) 735982..736308 + 327 WP_006479527.1 anthranilate 1,2-dioxygenase ferredoxin subunit AndAb -
DT99_RS20015 (DT99_020015) 736298..737518 + 1221 WP_034185658.1 anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase -
DT99_RS20020 (DT99_020020) 737636..738922 + 1287 WP_034185659.1 DUF445 domain-containing protein -
DT99_RS20025 (DT99_020025) 739006..739305 - 300 WP_034185660.1 putative addiction module antidote protein Antitoxin
DT99_RS20030 (DT99_020030) 739309..739647 - 339 WP_034185661.1 addiction module protein Toxin
DT99_RS20035 (DT99_020035) 739986..741626 + 1641 WP_034185662.1 acetolactate synthase large subunit -
DT99_RS20040 (DT99_020040) 741656..743089 + 1434 WP_034185663.1 aldehyde dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(16-109)

Antitoxin

Uncharacterized protein HI1420

(6-93)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12428.32 Da        Isoelectric Point: 10.1620

>T197960 WP_034185661.1 NZ_CP072521:c739647-739309 [Burkholderia seminalis]
MVGILPSAPYRAPTFSIRTTDVFDAWFAGLQDRVAKRRIQARIDCLSMGNPGDWKAAGSPVVEMRIDHGPGYRVYSVRRG
PIRVILRCGGDQSTQQADIRAAHAMLARLDME

Download         Length: 339 bp

>T197960 NZ_CP096837:2636239-2636346 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10676.12 Da        Isoelectric Point: 6.4777

>AT197960 WP_034185660.1 NZ_CP072521:c739305-739006 [Burkholderia seminalis]
MDKIRTRPWDSAEPLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Download         Length: 300 bp

>AT197960 NZ_CP096837:c2636192-2636126 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A8DBW7

Antitoxin

Source ID Structure

References