Detailed information of TA system TA197957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 38911..39433
• Replicon: plasmid p3Z-5L-3
• Accession: NZ_CP072518
• Organism: Klebsiella quasipneumoniae strain 3Z-5L

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: J6325_RS27790
• Protein ID: WP_004187019.1
• Coordinates: 39149..39433 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: J6325_RS27785
• Protein ID: WP_004187025.1
• Coordinates: 38911..39159 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6325_RS27755 (J6325_27610)	36278..36847	-	570	WP_004152117.1	small heat shock protein sHSP20	-
J6325_RS27760 (J6325_27615)	36882..37163	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
J6325_RS27765 (J6325_27620)	37407..37670	-	264	WP_004118208.1	hypothetical protein	-
J6325_RS27770 (J6325_27625)	37685..37948	+	264	WP_004187027.1	transposase	-
J6325_RS27775 (J6325_27630)	37893..38009	+	117	Protein_45	transposase domain-containing protein	-
J6325_RS27780 (J6325_27635)	38197..38621	+	425	Protein_46	IS1 family transposase	-
J6325_RS27785 (J6325_27640)	38911..39159	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
J6325_RS27790 (J6325_27645)	39149..39433	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6325_RS27795 (J6325_27650)	39449..40804	-	1356	WP_004187017.1	ISNCY family transposase	-
J6325_RS27800 (J6325_27655)	40886..41137	-	252	Protein_50	conjugal transfer protein TraH	-
J6325_RS27805 (J6325_27665)	41794..42207	-	414	WP_004187013.1	hypothetical protein	-
J6325_RS27810 (J6325_27670)	43750..44214	+	465	WP_004187010.1	conjugative transfer protein MobI(A/C)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D)	mrkJ / mrkF / mrkC / mrkB / mrkA	1..110830	110830
	-	flank	IS/Tn	-	-	38391..38657	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T197957 WP_004187019.1 NZ_CP072518:39149-39433 [Klebsiella quasipneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T197957 NZ_CP096837:2026805-2026907 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT197957 WP_004187025.1 NZ_CP072518:38911-39159 [Klebsiella quasipneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT197957 NZ_CP096837:c2026945-2026674 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTAAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4