Detailed information of TA system TA197956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 25280..26016
• Replicon: plasmid p3Z-5L-3
• Accession: NZ_CP072518
• Organism: Klebsiella quasipneumoniae strain 3Z-5L

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: J6325_RS27695
• Protein ID: WP_004187044.1
• Coordinates: 25280..25762 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: J6325_RS27700
• Protein ID: WP_003026799.1
• Coordinates: 25750..26016 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6325_RS27670 (J6325_27530)	21304..22240	-	937	Protein_24	ISNCY family transposase	-
J6325_RS27675 (J6325_27535)	22350..22499	+	150	Protein_25	transposase	-
J6325_RS27680 (J6325_27540)	22515..22625	+	111	Protein_26	IS3 family transposase	-
J6325_RS27685 (J6325_27545)	22859..23563	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
J6325_RS27690 (J6325_27550)	23723..25069	-	1347	WP_077251107.1	ISNCY family transposase	-
J6325_RS27695 (J6325_27555)	25280..25762	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
J6325_RS27700 (J6325_27560)	25750..26016	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
J6325_RS27705 (J6325_27565)	26167..26871	+	705	WP_060579418.1	IS6-like element IS26 family transposase	-
J6325_RS27710 (J6325_27570)	27030..29615	-	2586	WP_004187040.1	EAL domain-containing protein	-
J6325_RS27715 (J6325_27575)	29584..29829	-	246	WP_032238678.1	hypothetical protein	-
J6325_RS27720 (J6325_27580)	29887..30867	-	981	Protein_34	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D)	mrkJ / mrkF / mrkC / mrkB / mrkA	1..110830	110830
	-	inside	IScluster/Tn	-	-	21283..30867	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T197956 WP_004187044.1 NZ_CP072518:c25762-25280 [Klebsiella quasipneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T197956 NZ_CP096837:c1167593-1167273 [Escherichia coli]
ATGAAAACCCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCAGTAGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTTAGACAGCACTATGGCCTGACACTGAACGACACGCCGTTCAGTAATGAAACTGTTATTAAGGAACATATCG
ATGCCGGTATCTCTTTAGTCGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGCTCGCATCGATAGTAAGGGGTTT
AGCTGGCAAGAACAGTCGCCTTTTATTACTGCTGTAGACATTCTAAAAGCCAGACGGGCTATGAGGGAAATGAGAATTTA
A

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT197956 WP_003026799.1 NZ_CP072518:c26016-25750 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT197956 NZ_CP096837:c1167931-1167614 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGAGGGATATCACGCCGTGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTCGATGACCGGGCCAGTATCACCGGTAAGTTTAGTGATGCCCAATGTATTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGGTGCTGACCACTGGCGAACTGAATCCCCGTCATGCCCACTGCGTTACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66