Detailed information of TA system TA197955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 38195..39105
• Replicon: plasmid p3Z-5L-1
• Accession: NZ_CP072516
• Organism: Klebsiella quasipneumoniae strain 3Z-5L

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: J6325_RS25330
• Protein ID: WP_004181896.1
• Coordinates: 38195..38665 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: J6325_RS25335
• Protein ID: WP_004181895.1
• Coordinates: 38662..39105 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6325_RS25305 (J6325_25205)	34792..35169	+	378	WP_004181901.1	hypothetical protein	-
J6325_RS25310 (J6325_25210)	35367..36341	-	975	WP_040222313.1	hypothetical protein	-
J6325_RS28520 (J6325_25215)	36944..37102	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
J6325_RS25325 (J6325_25220)	37825..38085	+	261	WP_004026352.1	hypothetical protein	-
J6325_RS25330 (J6325_25225)	38195..38665	-	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
J6325_RS25335 (J6325_25230)	38662..39105	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
J6325_RS25340 (J6325_25235)	39206..40477	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
J6325_RS25345 (J6325_25240)	40477..40899	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
J6325_RS25350 (J6325_25245)	40985..42472	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
J6325_RS25355 (J6325_25250)	43164..43733	-	570	Protein_40	transposase	-
J6325_RS25360 (J6325_25255)	43735..43950	+	216	Protein_41	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB4 / blaDHA-1 / sul1 / aph(3'')-Ib / aph(6)-Id / dfrA12 / aadA2 / floR / sul2 / tet(A)	-	1..259774	259774

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T197955 WP_004181896.1 NZ_CP072516:c38665-38195 [Klebsiella quasipneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T197955 NZ_CP096837:1029115-1029450 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT197955 WP_004181895.1 NZ_CP072516:c39105-38662 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT197955 NZ_CP096837:1028867-1029115 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9