Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 6664..7244 Replicon plasmid unnamed3
Accession NZ_CP072499
Organism Klebsiella pneumoniae strain KPN247

Toxin (Protein)


Gene name parE Uniprot ID A0A060VNB1
Locus tag J5253_RS26965 Protein ID WP_001136729.1
Coordinates 6930..7244 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2X1PRM1
Locus tag J5253_RS26960 Protein ID WP_000093040.1
Coordinates 6664..6942 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5253_RS26930 (J5253_26925) 2704..2853 - 150 WP_032440454.1 colicin release lysis protein -
J5253_RS26935 (J5253_26930) 2938..3195 - 258 WP_032440455.1 colicin E3-like toxin immunity protein -
J5253_RS26940 (J5253_26935) 3205..4890 - 1686 WP_032440457.1 colicin-like bacteriocin tRNase domain-containing protein -
J5253_RS26945 (J5253_26940) 5217..5462 + 246 WP_159210150.1 hypothetical protein -
J5253_RS26950 (J5253_26945) 5736..6107 + 372 WP_001237044.1 cell envelope integrity protein TolA -
J5253_RS26955 (J5253_26950) 6104..6322 + 219 WP_002193751.1 TonB family protein -
J5253_RS26960 (J5253_26955) 6664..6942 + 279 WP_000093040.1 CopG family ribbon-helix-helix protein Antitoxin
J5253_RS26965 (J5253_26960) 6930..7244 + 315 WP_001136729.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J5253_RS26970 (J5253_26965) 7408..7836 + 429 WP_001140599.1 hypothetical protein -
J5253_RS26975 (J5253_26970) 7862..8041 + 180 WP_065521055.1 Rop family plasmid primer RNA-binding protein -
J5253_RS26980 (J5253_26975) 8069..8599 - 531 WP_159210149.1 hypothetical protein -
J5253_RS26985 (J5253_26980) 8606..9337 - 732 WP_159210148.1 MobC family replication-relaxation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..10077 10077


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-96)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11847.77 Da        Isoelectric Point: 9.8619

>T197936 WP_001136729.1 NZ_CP072499:6930-7244 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKTLPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDR
EMDRIVIVTVRHQKESGYPGADSL

Download         Length: 315 bp

>T197936 NZ_CP096834:3037850-3038080 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10709.73 Da        Isoelectric Point: 4.7561

>AT197936 WP_000093040.1 NZ_CP072499:6664-6942 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Download         Length: 279 bp

>AT197936 NZ_CP096834:3038077-3038436 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VNB1

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1PRM1

References