Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3144..3787 Replicon plasmid unnamed2
Accession NZ_CP072498
Organism Klebsiella pneumoniae strain KPN247

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag J5253_RS26620 Protein ID WP_000754566.1
Coordinates 3144..3560 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag J5253_RS26625 Protein ID WP_001261276.1
Coordinates 3557..3787 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5253_RS26605 (J5253_26600) 2..85 - 84 Protein_0 SOS response-associated peptidase -
J5253_RS27215 605..706 + 102 Protein_1 Tn3 family transposase -
J5253_RS26610 (J5253_26605) 726..1802 - 1077 WP_000227969.1 IS110 family transposase -
J5253_RS26615 (J5253_26610) 2084..2940 - 857 Protein_3 IS3-like element ISEc15 family transposase -
J5253_RS26620 (J5253_26615) 3144..3560 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
J5253_RS26625 (J5253_26620) 3557..3787 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J5253_RS26630 (J5253_26625) 4361..4711 + 351 WP_000493378.1 hypothetical protein -
J5253_RS26635 (J5253_26630) 4762..5505 + 744 WP_000129823.1 hypothetical protein -
J5253_RS26640 (J5253_26635) 5502..6278 + 777 WP_000015958.1 site-specific integrase -
J5253_RS26645 (J5253_26640) 6336..6593 - 258 WP_000764642.1 hypothetical protein -
J5253_RS26650 (J5253_26645) 6722..6826 - 105 WP_032409716.1 hypothetical protein -
J5253_RS26655 (J5253_26650) 7356..8222 + 867 WP_004118283.1 replication initiation protein -
J5253_RS26660 (J5253_26655) 8399..8668 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / floR / tet(A) / sul1 / qacE / aadA2 / dfrA12 / qnrS1 / aph(3'')-Ib / sul2 - 1..55508 55508


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T197935 WP_000754566.1 NZ_CP072498:c3560-3144 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T197935 NZ_CP096834:2963448-2963555 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT197935 WP_001261276.1 NZ_CP072498:c3787-3557 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT197935 NZ_CP096834:c2963391-2963336 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References