Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5245604..5246229 Replicon chromosome
Accession NZ_CP072496
Organism Klebsiella pneumoniae strain KPN247

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J5253_RS25550 Protein ID WP_002882817.1
Coordinates 5245604..5245987 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J5253_RS25555 Protein ID WP_004150355.1
Coordinates 5245987..5246229 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5253_RS25535 (J5253_25530) 5242970..5243872 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J5253_RS25540 (J5253_25535) 5243869..5244504 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J5253_RS25545 (J5253_25540) 5244501..5245430 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J5253_RS25550 (J5253_25545) 5245604..5245987 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J5253_RS25555 (J5253_25550) 5245987..5246229 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J5253_RS25560 (J5253_25555) 5246434..5247351 + 918 WP_023302328.1 alpha/beta hydrolase -
J5253_RS25565 (J5253_25560) 5247365..5248306 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J5253_RS25570 (J5253_25565) 5248351..5248788 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J5253_RS25575 (J5253_25570) 5248785..5249645 - 861 WP_004146232.1 virulence factor BrkB family protein -
J5253_RS25580 (J5253_25575) 5249639..5250238 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197934 WP_002882817.1 NZ_CP072496:c5245987-5245604 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197934 NZ_CP096834:2962965-2963072 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197934 WP_004150355.1 NZ_CP072496:c5246229-5245987 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197934 NZ_CP096834:c2962907-2962853 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References