Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4050492..4051111 Replicon chromosome
Accession NZ_CP072496
Organism Klebsiella pneumoniae strain KPN247

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J5253_RS19845 Protein ID WP_002892050.1
Coordinates 4050893..4051111 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag J5253_RS19840 Protein ID WP_002892066.1
Coordinates 4050492..4050866 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5253_RS19830 (J5253_19825) 4045644..4046837 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J5253_RS19835 (J5253_19830) 4046860..4050006 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
J5253_RS19840 (J5253_19835) 4050492..4050866 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
J5253_RS19845 (J5253_19840) 4050893..4051111 + 219 WP_002892050.1 HHA domain-containing protein Toxin
J5253_RS19850 (J5253_19845) 4051270..4051836 + 567 WP_002892030.1 maltose O-acetyltransferase -
J5253_RS19855 (J5253_19850) 4051808..4051948 - 141 WP_004147370.1 hypothetical protein -
J5253_RS19860 (J5253_19855) 4051969..4052439 + 471 WP_002892026.1 YlaC family protein -
J5253_RS19865 (J5253_19860) 4052414..4053865 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
J5253_RS19870 (J5253_19865) 4053966..4054664 + 699 WP_002892021.1 GNAT family protein -
J5253_RS19875 (J5253_19870) 4054661..4054801 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J5253_RS19880 (J5253_19875) 4054801..4055064 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197932 WP_002892050.1 NZ_CP072496:4050893-4051111 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197932 NZ_CP096834:c2290500-2290327 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT197932 WP_002892066.1 NZ_CP072496:4050492-4050866 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197932 NZ_CP096834:c2290824-2290528 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References