Detailed information of TA system TA197931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2729751..2731339
• Replicon: chromosome
• Accession: NZ_CP072496
• Organism: Klebsiella pneumoniae strain KPN247

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A485W5A2
• Locus tag: J5253_RS13410
• Protein ID: WP_023342812.1
• Coordinates: 2729751..2731073 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J5253_RS13415
• Protein ID: WP_024622638.1
• Coordinates: 2731073..2731339 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5253_RS13395 (J5253_13395)	2724807..2727308	+	2502	WP_023342811.1	fimbria/pilus outer membrane usher protein	-
J5253_RS13400 (J5253_13400)	2727296..2728300	+	1005	WP_004224758.1	fimbrial protein	-
J5253_RS13405 (J5253_13405)	2728442..2729734	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J5253_RS13410 (J5253_13410)	2729751..2731073	-	1323	WP_023342812.1	type II toxin-antitoxin system HipA family toxin	Toxin
J5253_RS13415 (J5253_13415)	2731073..2731339	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J5253_RS13420 (J5253_13420)	2731604..2731930	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
J5253_RS13425 (J5253_13425)	2731927..2732427	-	501	WP_020804988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J5253_RS13430 (J5253_13430)	2732405..2733784	-	1380	WP_048292036.1	nucleobase:cation symporter-2 family protein	-
J5253_RS13435 (J5253_13435)	2734130..2735284	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J5253_RS13440 (J5253_13440)	2735265..2736188	-	924	WP_040089016.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5358

>T197931 WP_023342812.1 NZ_CP072496:c2731073-2729751 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEVNAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T197931 NZ_CP096834:c2186948-2186598 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTTGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT197931 WP_024622638.1 NZ_CP072496:c2731339-2731073 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT197931 NZ_CP096834:c2187193-2186942 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A485W5A2