Detailed information of TA system TA197922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1879041..1879684
• Replicon: chromosome
• Accession: NZ_CP072496
• Organism: Klebsiella pneumoniae strain KPN247

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: J5253_RS09165
• Protein ID: WP_015874977.1
• Coordinates: 1879041..1879457 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: J5253_RS09170
• Protein ID: WP_015874976.1
• Coordinates: 1879454..1879684 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5253_RS09150 (J5253_09150)	1874444..1875559	-	1116	WP_032447702.1	salmochelin biosynthesis C-glycosyltransferase IroB	-
J5253_RS27020	1875732..1876010	-	279	Protein_1792	ISNCY family transposase	-
J5253_RS09155 (J5253_09155)	1876431..1878605	+	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
J5253_RS09160 (J5253_09160)	1878760..1879005	-	246	WP_032447699.1	hypothetical protein	-
J5253_RS09165 (J5253_09165)	1879041..1879457	-	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
J5253_RS09170 (J5253_09170)	1879454..1879684	-	231	WP_015874976.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J5253_RS09175 (J5253_09175)	1880127..1880507	+	381	WP_015874975.1	YrzE family protein	-
J5253_RS09180 (J5253_09180)	1880695..1881665	+	971	Protein_1798	IS21-like element IS100 family transposase	-
J5253_RS09185 (J5253_09185)	1881692..1882418	+	727	Protein_1799	IS21-like element helper ATPase IstB	-
J5253_RS09190 (J5253_09190)	1882462..1882575	-	114	WP_015874970.1	Hha/YmoA family nucleoid-associated regulatory protein	-
J5253_RS09195 (J5253_09195)	1882818..1883027	-	210	WP_032447696.1	TraR/DksA family transcriptional regulator	-
J5253_RS09200 (J5253_09200)	1883017..1883598	-	582	WP_000937857.1	DUF2857 domain-containing protein	-
J5253_RS09205 (J5253_09205)	1883583..1883765	-	183	WP_072001695.1	hypothetical protein	-
J5253_RS09210 (J5253_09210)	1883787..1883972	-	186	WP_000205185.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rmpA / iroD / iroC / iroB / iroN / fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / irp2 / ybtA / ybtP	1840167..1911545	71378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T197922 WP_015874977.1 NZ_CP072496:c1879457-1879041 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T197922 NZ_CP096834:52122-52225 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT197922 WP_015874976.1 NZ_CP072496:c1879684-1879454 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT197922 NZ_CP096834:c52263-51992 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4