Detailed information of TA system TA197918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 132686..133356
• Replicon: plasmid unnamed1
• Accession: NZ_CP072495
• Organism: Klebsiella pneumoniae strain KPN246

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: J7V84_RS26440
• Protein ID: WP_004213072.1
• Coordinates: 132686..133129 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: J7V84_RS26445
• Protein ID: WP_004213073.1
• Coordinates: 133126..133356 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V84_RS26405 (J7V84_26400)	128096..128371	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
J7V84_RS26410 (J7V84_26405)	128434..128925	+	492	WP_011251327.1	DM13 domain-containing protein	-
J7V84_RS26415 (J7V84_26410)	128974..129894	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
J7V84_RS26420 (J7V84_26415)	129985..130388	+	404	Protein_138	GAF domain-containing protein	-
J7V84_RS26425 (J7V84_26420)	130906..131542	-	637	Protein_139	mucoid phenotype regulator RmpA2	-
J7V84_RS26430 (J7V84_26425)	131959..132263	+	305	Protein_140	transposase	-
J7V84_RS27075	132286..132537	-	252	WP_186987481.1	hypothetical protein	-
J7V84_RS26440 (J7V84_26435)	132686..133129	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7V84_RS26445 (J7V84_26440)	133126..133356	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7V84_RS26450 (J7V84_26445)	133964..135097	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
J7V84_RS26455 (J7V84_26450)	135113..135406	+	294	WP_004213076.1	hypothetical protein	-
J7V84_RS26460 (J7V84_26455)	135396..135602	-	207	WP_004213077.1	hypothetical protein	-
J7V84_RS26465 (J7V84_26460)	135954..136244	+	291	WP_004213078.1	hypothetical protein	-
J7V84_RS26470 (J7V84_26465)	136234..137133	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..208189	208189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T197918 WP_004213072.1 NZ_CP072495:c133129-132686 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T197918 NZ_CP096824:2316418-2317155 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT197918 WP_004213073.1 NZ_CP072495:c133356-133126 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT197918 NZ_CP096824:2317268-2317393 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620