Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 67200..67936 Replicon plasmid unnamed1
Accession NZ_CP072495
Organism Klebsiella pneumoniae strain KPN246

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag J7V84_RS26105 Protein ID WP_004098919.1
Coordinates 67200..67682 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag J7V84_RS26110 Protein ID WP_004213599.1
Coordinates 67670..67936 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V84_RS26085 (J7V84_26080) 62554..63900 + 1347 WP_011154555.1 dihydroorotase -
J7V84_RS26090 (J7V84_26085) 63964..64986 + 1023 WP_004214536.1 porphobilinogen synthase -
J7V84_RS26095 (J7V84_26090) 65161..65556 + 396 Protein_73 IS3 family transposase -
J7V84_RS26100 (J7V84_26095) 65552..66779 - 1228 Protein_74 IS3 family transposase -
J7V84_RS26105 (J7V84_26100) 67200..67682 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
J7V84_RS26110 (J7V84_26105) 67670..67936 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
J7V84_RS26115 (J7V84_26110) 68131..68361 - 231 WP_004213598.1 hypothetical protein -
J7V84_RS26120 (J7V84_26115) 68375..68578 - 204 WP_004213596.1 HHA domain-containing protein -
J7V84_RS26125 (J7V84_26120) 68639..69133 - 495 WP_004213594.1 hypothetical protein -
J7V84_RS26130 (J7V84_26125) 69175..72144 - 2970 WP_004213592.1 Tn3 family transposase -
J7V84_RS26135 (J7V84_26130) 72147..72704 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..208189 208189
- inside IScluster/Tn - - 65552..72704 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T197917 WP_004098919.1 NZ_CP072495:c67682-67200 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T197917 NZ_CP096824:241073-241423 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT197917 WP_004213599.1 NZ_CP072495:c67936-67670 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT197917 NZ_CP096824:240781-241068 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References