Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 43609..44519 Replicon plasmid unnamed1
Accession NZ_CP072495
Organism Klebsiella pneumoniae strain KPN246

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag J7V84_RS25995 Protein ID WP_004026354.1
Coordinates 44049..44519 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag J7V84_RS25990 Protein ID WP_004026357.1
Coordinates 43609..44052 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V84_RS25960 (J7V84_25955) 38627..39148 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
J7V84_RS25965 (J7V84_25960) 39145..40098 + 954 Protein_45 fec operon regulator FecR -
J7V84_RS25970 (J7V84_25965) 40185..42311 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
J7V84_RS25975 (J7V84_25970) 42386..42505 + 120 Protein_47 Fe3+-citrate ABC transporter substrate-binding protein -
J7V84_RS25980 (J7V84_25975) 42686..42907 + 222 WP_004213643.1 DUF2188 domain-containing protein -
J7V84_RS25985 (J7V84_25980) 43005..43508 + 504 Protein_49 DUF4113 domain-containing protein -
J7V84_RS25990 (J7V84_25985) 43609..44052 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
J7V84_RS25995 (J7V84_25990) 44049..44519 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
J7V84_RS26000 (J7V84_25995) 45033..45730 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
J7V84_RS26005 (J7V84_26000) 47116..47766 - 651 WP_068893702.1 DUF1173 family protein -
J7V84_RS27065 47799..48065 - 267 WP_223175074.1 DUF1173 family protein -
J7V84_RS26010 (J7V84_26005) 48243..48737 + 495 WP_004212794.1 thermonuclease family protein -
J7V84_RS26015 (J7V84_26010) 49074..49472 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..208189 208189


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T197916 WP_004026354.1 NZ_CP072495:44049-44519 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T197916 NZ_CP096822:c5525550-5525272 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT197916 WP_004026357.1 NZ_CP072495:43609-44052 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT197916 NZ_CP096822:c5525260-5524955 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCTGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References