Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5244162..5244787 Replicon chromosome
Accession NZ_CP072494
Organism Klebsiella pneumoniae strain KPN246

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J7V84_RS25390 Protein ID WP_002882817.1
Coordinates 5244162..5244545 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J7V84_RS25395 Protein ID WP_004150355.1
Coordinates 5244545..5244787 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V84_RS25375 (5241528) 5241528..5242430 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J7V84_RS25380 (5242427) 5242427..5243062 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J7V84_RS25385 (5243059) 5243059..5243988 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J7V84_RS25390 (5244162) 5244162..5244545 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J7V84_RS25395 (5244545) 5244545..5244787 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J7V84_RS25400 (5244992) 5244992..5245909 + 918 WP_088497967.1 alpha/beta hydrolase -
J7V84_RS25405 (5245923) 5245923..5246864 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J7V84_RS25410 (5246909) 5246909..5247346 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J7V84_RS25415 (5247343) 5247343..5248203 - 861 WP_002882807.1 virulence factor BrkB family protein -
J7V84_RS25420 (5248197) 5248197..5248796 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197915 WP_002882817.1 NZ_CP072494:c5244545-5244162 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197915 NZ_CP096822:c4971082-4970735 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197915 WP_004150355.1 NZ_CP072494:c5244787-5244545 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197915 NZ_CP096822:c4971343-4971092 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References