Detailed information of TA system TA197898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 156062..156732
• Replicon: plasmid unnamed1
• Accession: NZ_CP072493
• Organism: Klebsiella pneumoniae strain KPN236

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: J7V87_RS26610
• Protein ID: WP_063938211.1
• Coordinates: 156062..156505 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: J7V87_RS26615
• Protein ID: WP_004213073.1
• Coordinates: 156502..156732 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V87_RS26575 (J7V87_26570)	151466..151741	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
J7V87_RS26580 (J7V87_26575)	151804..152295	+	492	WP_011251327.1	DM13 domain-containing protein	-
J7V87_RS26585 (J7V87_26580)	152344..153264	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
J7V87_RS26590 (J7V87_26585)	153355..153758	+	404	Protein_168	GAF domain-containing protein	-
J7V87_RS26595 (J7V87_26590)	154276..154917	-	642	Protein_169	mucoid phenotype regulator RmpA2	-
J7V87_RS26600 (J7V87_26595)	155335..155639	+	305	Protein_170	transposase	-
J7V87_RS27165	155662..155913	-	252	WP_186987481.1	hypothetical protein	-
J7V87_RS26610 (J7V87_26605)	156062..156505	-	444	WP_063938211.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7V87_RS26615 (J7V87_26610)	156502..156732	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7V87_RS26620 (J7V87_26615)	157340..158473	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
J7V87_RS26625 (J7V87_26620)	158489..158782	+	294	WP_004213076.1	hypothetical protein	-
J7V87_RS26630 (J7V87_26625)	158772..158978	-	207	WP_004213077.1	hypothetical protein	-
J7V87_RS26635 (J7V87_26630)	159330..159620	+	291	WP_004213078.1	hypothetical protein	-
J7V87_RS26640 (J7V87_26635)	159610..160509	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..231163	231163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16036.77 Da
• Isoelectric Point: 9.3642

>T197898 WP_063938211.1 NZ_CP072493:c156505-156062 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCTRLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T197898 NZ_CP096818:c2459567-2459385 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATAGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACGT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT197898 WP_004213073.1 NZ_CP072493:c156732-156502 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT197898 NZ_CP096818:c2459292-2458888 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Antitoxin

• AlphaFold DB: Q6U620