Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5319568..5320193 Replicon chromosome
Accession NZ_CP072492
Organism Klebsiella pneumoniae strain KPN236

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag J7V87_RS25405 Protein ID WP_012737091.1
Coordinates 5319568..5319951 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J7V87_RS25410 Protein ID WP_004150355.1
Coordinates 5319951..5320193 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V87_RS25390 (5316934) 5316934..5317836 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J7V87_RS25395 (5317833) 5317833..5318468 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J7V87_RS25400 (5318465) 5318465..5319394 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J7V87_RS25405 (5319568) 5319568..5319951 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
J7V87_RS25410 (5319951) 5319951..5320193 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J7V87_RS25415 (5320398) 5320398..5321315 + 918 WP_012737090.1 alpha/beta hydrolase -
J7V87_RS25420 (5321329) 5321329..5322270 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J7V87_RS25425 (5322315) 5322315..5322752 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J7V87_RS25430 (5322749) 5322749..5323609 - 861 WP_004146232.1 virulence factor BrkB family protein -
J7V87_RS25435 (5323603) 5323603..5324202 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T197895 WP_012737091.1 NZ_CP072492:c5319951-5319568 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197895 NZ_CP096815:c121460-120978 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197895 WP_004150355.1 NZ_CP072492:c5320193-5319951 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197895 NZ_CP096815:c121714-121448 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References