Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4037020..4037639 Replicon chromosome
Accession NZ_CP072492
Organism Klebsiella pneumoniae strain KPN236

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J7V87_RS19420 Protein ID WP_002892050.1
Coordinates 4037020..4037238 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag J7V87_RS19425 Protein ID WP_002892066.1
Coordinates 4037265..4037639 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V87_RS19385 (4033067) 4033067..4033330 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
J7V87_RS19390 (4033330) 4033330..4033470 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J7V87_RS19395 (4033467) 4033467..4034165 - 699 WP_002892021.1 GNAT family protein -
J7V87_RS19400 (4034266) 4034266..4035717 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
J7V87_RS19405 (4035692) 4035692..4036162 - 471 WP_002892026.1 YlaC family protein -
J7V87_RS19410 (4036183) 4036183..4036323 + 141 WP_004147370.1 hypothetical protein -
J7V87_RS19415 (4036295) 4036295..4036861 - 567 WP_002892030.1 maltose O-acetyltransferase -
J7V87_RS19420 (4037020) 4037020..4037238 - 219 WP_002892050.1 HHA domain-containing protein Toxin
J7V87_RS19425 (4037265) 4037265..4037639 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
J7V87_RS19430 (4038125) 4038125..4041271 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
J7V87_RS19435 (4041294) 4041294..4042487 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197892 WP_002892050.1 NZ_CP072492:c4037238-4037020 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197892 NZ_CP096814:c4756401-4756117 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT197892 WP_002892066.1 NZ_CP072492:c4037639-4037265 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197892 NZ_CP096814:c4756633-4756391 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References