Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 104248..104987 Replicon plasmid p1B6885
Accession NZ_CP072469
Organism Klebsiella quasipneumoniae strain B6885

Toxin (Protein)


Gene name tacT Uniprot ID J6I5J9
Locus tag J6315_RS26685 Protein ID WP_009653238.1
Coordinates 104248..104733 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID J5VA20
Locus tag J6315_RS26690 Protein ID WP_009653219.1
Coordinates 104721..104987 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6315_RS26645 (J6315_26640) 99428..99748 - 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
J6315_RS26650 (J6315_26645) 99829..100053 - 225 WP_004152719.1 hypothetical protein -
J6315_RS26655 (J6315_26650) 100064..100276 - 213 WP_004152718.1 hypothetical protein -
J6315_RS26660 (J6315_26655) 100337..100693 - 357 WP_004152717.1 hypothetical protein -
J6315_RS26665 (J6315_26660) 101329..101679 - 351 WP_023280874.1 hypothetical protein -
J6315_RS26670 (J6315_26665) 101676..101948 - 273 WP_023280873.1 hypothetical protein -
J6315_RS27465 (J6315_26670) 102644..102805 - 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
J6315_RS26680 (J6315_26675) 102875..103954 + 1080 WP_009653230.1 IS481 family transposase -
J6315_RS26685 (J6315_26680) 104248..104733 - 486 WP_009653238.1 GNAT family N-acetyltransferase Toxin
J6315_RS26690 (J6315_26685) 104721..104987 - 267 WP_009653219.1 DUF1778 domain-containing protein Antitoxin
J6315_RS26695 (J6315_26690) 105215..105592 + 378 WP_196239799.1 hypothetical protein -
J6315_RS26700 (J6315_26695) 105637..106374 + 738 WP_009654087.1 HupE/UreJ family protein -
J6315_RS26705 (J6315_26700) 106388..107077 + 690 WP_009654088.1 hypothetical protein -
J6315_RS26710 (J6315_26705) 107218..107589 + 372 WP_029602876.1 hypothetical protein -
J6315_RS26715 (J6315_26710) 107653..108573 + 921 WP_009651631.1 hypothetical protein -
J6315_RS26720 (J6315_26715) 108627..109385 + 759 WP_023303135.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..134890 134890
- flank IS/Tn - - 102875..103954 1079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-150)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17756.43 Da        Isoelectric Point: 9.9000

>T197878 WP_009653238.1 NZ_CP072469:c104733-104248 [Klebsiella quasipneumoniae]
VGYVTSPEPLSSFHQVAEFISGEAVLDDWLKQRSLKNQATGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCFRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQSQPRTLFLRLP
Q

Download         Length: 486 bp

>T197878 NZ_CP096813:c321769-321491 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10123.58 Da        Isoelectric Point: 4.4128

>AT197878 WP_009653219.1 NZ_CP072469:c104987-104721 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDEQYAEFIDMIDAPVADDPAIEKLL
ARKPQWDM

Download         Length: 267 bp

>AT197878 NZ_CP096813:c321479-321174 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A142I4R1

Antitoxin

Source ID Structure
AlphaFold DB A0A142I4R0

References