Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 85543..86068 Replicon plasmid p1B6885
Accession NZ_CP072469
Organism Klebsiella quasipneumoniae strain B6885

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag J6315_RS26555 Protein ID WP_001568026.1
Coordinates 85763..86068 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag J6315_RS26550 Protein ID WP_001568025.1
Coordinates 85543..85761 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6315_RS26520 (J6315_26515) 81179..82576 - 1398 WP_032452481.1 diguanylate cyclase -
J6315_RS26530 (J6315_26525) 83549..84238 - 690 Protein_81 AAA family ATPase -
J6315_RS26535 (J6315_26530) 84312..84728 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
J6315_RS26540 (J6315_26535) 84725..84955 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
J6315_RS26545 (J6315_26540) 84912..85373 + 462 Protein_84 hypothetical protein -
J6315_RS26550 (J6315_26545) 85543..85761 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
J6315_RS26555 (J6315_26550) 85763..86068 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
J6315_RS26560 (J6315_26555) 86237..86632 + 396 WP_072158608.1 hypothetical protein -
J6315_RS26565 (J6315_26560) 86659..86982 + 324 WP_022644730.1 hypothetical protein -
J6315_RS26570 (J6315_26565) 86979..87995 + 1017 WP_020315256.1 hypothetical protein -
J6315_RS26575 (J6315_26570) 88148..88852 + 705 WP_001067834.1 IS6-like element IS26 family transposase -
J6315_RS26580 (J6315_26575) 88906..89280 - 375 Protein_91 DUF86 domain-containing protein -
J6315_RS26585 (J6315_26580) 89277..89567 - 291 WP_001247892.1 nucleotidyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..134890 134890
- inside IScluster/Tn - - 70045..92530 22485


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T197877 WP_001568026.1 NZ_CP072469:85763-86068 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T197877 NZ_CP096812:c1845788-1845525 [Acinetobacter baumannii]
ATGAACGTATTAACTTATACGGATGCTCGTAAAAATCTAAAATCTATTTTAGATCAAGTAGTTGATGATGCAGATATAAC
TATTATTACTAGGCGTGAAGGTGGGAATGCAGTTGTAATGGGCCAGGACTATTATAATAGGCTTATGGAAACATTACACC
TTATGTCCTCACCTAGTAATGCAGCGCATCTTGCAAAATCAATTGCTGAGTTACGAGCATCTAAAGCTATTGAGAGAGAG
CTTTTAGATGACGAGAAAGATTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT197877 WP_001568025.1 NZ_CP072469:85543-85761 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT197877 NZ_CP096812:c1845541-1845278 [Acinetobacter baumannii]
ATGACGAGAAAGATTAGTTTTACACCTTCGGCGTGGGAAACTTATATGTTTTGGCAAAGCCAGGACAGAAAAACTTTAAA
GCGTATTAATCTTCTGATCAAGGATTGTTTACGCTCACCCTTTGAAGGTTTGGGAAAGCCTGAAGCTCTAATCGGTGATT
TATCAGGATTTTGGAGCAGACGAATTGATGAAAAGAATCGTCTTGTCTATGAAGTTACTGATTCAGCAATTACGATTCTA
TCGTGTCGCTATCATTATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References