Detailed information of TA system TA197862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1254637..1255286
• Replicon: chromosome
• Accession: NZ_CP072467
• Organism: Spiractinospora alimapuensis strain VN6-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J4H86_RS05790
• Protein ID: WP_236542471.1
• Coordinates: 1254637..1255056 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J4H86_RS05795
• Protein ID: WP_236542472.1
• Coordinates: 1255053..1255286 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4H86_RS05780 (J4H86_05750)	1252150..1252602	-	453	WP_236542469.1	GreA/GreB family elongation factor	-
J4H86_RS05785 (J4H86_05755)	1253036..1254619	+	1584	WP_236542470.1	helix-turn-helix domain-containing protein	-
J4H86_RS05790 (J4H86_05760)	1254637..1255056	-	420	WP_236542471.1	type II toxin-antitoxin system VapC family toxin	Toxin
J4H86_RS05795 (J4H86_05765)	1255053..1255286	-	234	WP_236542472.1	toxin-antitoxin system	Antitoxin
J4H86_RS05800 (J4H86_05770)	1255342..1256016	-	675	WP_236542473.1	DUF1028 domain-containing protein	-
J4H86_RS05805 (J4H86_05775)	1256109..1256330	+	222	WP_236542474.1	DUF2945 domain-containing protein	-
J4H86_RS05810 (J4H86_05780)	1256339..1256887	+	549	WP_236542475.1	DUF488 domain-containing protein	-
J4H86_RS05815 (J4H86_05785)	1256963..1257484	+	522	WP_236542476.1	helix-turn-helix domain-containing protein	-
J4H86_RS05820 (J4H86_05790)	1257481..1258341	+	861	WP_236542477.1	M23 family metallopeptidase	-
J4H86_RS05825 (J4H86_05795)	1258607..1259977	+	1371	WP_236542478.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15229.35 Da
• Isoelectric Point: 4.6381

>T197862 WP_236542471.1 NZ_CP072467:c1255056-1254637 [Spiractinospora alimapuensis]
MIVLDTNVISEIFRPNPDERVVTWLGSLTGEVAVTAVTLAELRAGVGRLPEGKRRSALQMRIEAALQPYRETRAILVFDD
DAAGHYAEIVVARERAGIPISMADAQIAAMCRSNSATCATRNTKDFTETGIELINPWDE

Nucleotide

• Length: 420 bp

>T197862 NZ_CP096810:3615058-3615426 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GCCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8235.55 Da
• Isoelectric Point: 6.4613

>AT197862 WP_236542472.1 NZ_CP072467:c1255286-1255053 [Spiractinospora alimapuensis]
MASIVVRGLDESVKKQIAIQAKEHGHSMEAEVREILTRAAGRPNIGIALMRAVMDVGGVDDLPVPVRTDAARVVDFG

Nucleotide

• Length: 234 bp

>AT197862 NZ_CP096810:3614840-3615061 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA