Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54461..55104 Replicon plasmid unnamed2
Accession NZ_CP072465
Organism Klebsiella pneumoniae strain KP1517

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HRG83_RS28780 Protein ID WP_001044770.1
Coordinates 54688..55104 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HRG83_RS28775 Protein ID WP_001261282.1
Coordinates 54461..54691 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRG83_RS28740 (49684) 49684..50463 - 780 WP_013214009.1 site-specific integrase -
HRG83_RS28745 (50647) 50647..51651 - 1005 WP_011977814.1 hypothetical protein -
HRG83_RS28750 (51681) 51681..51884 - 204 WP_011977813.1 hypothetical protein -
HRG83_RS28755 (51930) 51930..52451 - 522 WP_013214008.1 hypothetical protein -
HRG83_RS28760 (52509) 52509..52901 - 393 WP_011977811.1 hypothetical protein -
HRG83_RS28765 (52938) 52938..53882 - 945 WP_011977810.1 hypothetical protein -
HRG83_RS28770 (54043) 54043..54504 - 462 WP_014343465.1 hypothetical protein -
HRG83_RS28775 (54461) 54461..54691 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HRG83_RS28780 (54688) 54688..55104 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HRG83_RS28785 (55178) 55178..56740 + 1563 WP_004206609.1 AAA family ATPase -
HRG83_RS28790 (56725) 56725..57747 + 1023 WP_000361404.1 helicase UvrD -
HRG83_RS28795 (58003) 58003..58700 + 698 WP_223174630.1 IS1 family transposase -
HRG83_RS28800 (58729) 58729..59001 + 273 Protein_82 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid fosA3 / rmtB / blaSHV-12 / blaKPC-2 - 1..119109 119109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T197853 WP_001044770.1 NZ_CP072465:54688-55104 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T197853 NZ_CP096810:c1830143-1829733 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGCATGCTGCTGCGTTTGCGTCATGTCGATAACCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT197853 WP_001261282.1 NZ_CP072465:54461-54691 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT197853 NZ_CP096810:c1830390-1830124 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References