Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5543449..5544074 Replicon chromosome
Accession NZ_CP072463
Organism Klebsiella pneumoniae strain KP1517

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HRG83_RS27435 Protein ID WP_002882817.1
Coordinates 5543449..5543832 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HRG83_RS27440 Protein ID WP_004150355.1
Coordinates 5543832..5544074 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRG83_RS27420 (HRG83_27415) 5540815..5541717 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HRG83_RS27425 (HRG83_27420) 5541714..5542349 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HRG83_RS27430 (HRG83_27425) 5542346..5543275 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HRG83_RS27435 (HRG83_27430) 5543449..5543832 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HRG83_RS27440 (HRG83_27435) 5543832..5544074 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HRG83_RS27445 (HRG83_27440) 5544279..5545196 + 918 WP_002882812.1 alpha/beta hydrolase -
HRG83_RS27450 (HRG83_27445) 5545210..5546151 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HRG83_RS27455 (HRG83_27450) 5546196..5546633 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HRG83_RS27460 (HRG83_27455) 5546630..5547490 - 861 WP_002882807.1 virulence factor BrkB family protein -
HRG83_RS27465 (HRG83_27460) 5547484..5548083 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T197852 WP_002882817.1 NZ_CP072463:c5543832-5543449 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197852 NZ_CP096810:452250-452352 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197852 WP_004150355.1 NZ_CP072463:c5544074-5543832 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197852 NZ_CP096810:c452359-452214 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References