Detailed information of TA system TA197850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5079094..5079764
• Replicon: chromosome
• Accession: NZ_CP072463
• Organism: Klebsiella pneumoniae strain KP1517

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HRG83_RS25145
• Protein ID: WP_004213072.1
• Coordinates: 5079094..5079537 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HRG83_RS25150
• Protein ID: WP_004213073.1
• Coordinates: 5079534..5079764 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRG83_RS25110 (HRG83_25105)	5074514..5074780	+	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-
HRG83_RS25115 (HRG83_25110)	5074843..5075334	+	492	WP_011251327.1	DM13 domain-containing protein	-
HRG83_RS25120 (HRG83_25115)	5075383..5076303	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HRG83_RS25125 (HRG83_25120)	5076394..5076797	+	404	Protein_4940	GAF domain-containing protein	-
HRG83_RS25130 (HRG83_25125)	5077315..5077950	-	636	WP_165475474.1	mucoid phenotype regulator RmpA2	-
HRG83_RS25135 (HRG83_25130)	5078367..5078671	+	305	Protein_4942	transposase	-
HRG83_RS29470	5078694..5078945	-	252	WP_186987481.1	hypothetical protein	-
HRG83_RS25145 (HRG83_25140)	5079094..5079537	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HRG83_RS25150 (HRG83_25145)	5079534..5079764	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HRG83_RS25155 (HRG83_25150)	5080372..5081505	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HRG83_RS25160 (HRG83_25155)	5081521..5081814	+	294	WP_004213076.1	hypothetical protein	-
HRG83_RS25165 (HRG83_25160)	5081804..5082010	-	207	WP_004213077.1	hypothetical protein	-
HRG83_RS25170 (HRG83_25165)	5082362..5082652	+	291	WP_004213078.1	hypothetical protein	-
HRG83_RS25175 (HRG83_25170)	5082642..5083541	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	rmpA	5077315..5112001	34686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T197850 WP_004213072.1 NZ_CP072463:c5079537-5079094 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T197850 NZ_CP096809:925918-926172 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT197850 WP_004213073.1 NZ_CP072463:c5079764-5079534 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT197850 NZ_CP096809:925674-925916 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620