Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 5000873..5001399 Replicon chromosome
Accession NZ_CP072463
Organism Klebsiella pneumoniae strain KP1517

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HRG83_RS24745 Protein ID WP_000323025.1
Coordinates 5001112..5001399 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HRG83_RS24740 Protein ID WP_000534858.1
Coordinates 5000873..5001112 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRG83_RS24715 (HRG83_24710) 4995935..4996639 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HRG83_RS24720 (HRG83_24715) 4996871..4998211 + 1341 WP_000589001.1 ISNCY family transposase -
HRG83_RS24725 (HRG83_24720) 4998632..4998943 + 312 Protein_4859 transposase -
HRG83_RS24735 (HRG83_24730) 5000287..5000610 + 324 WP_224948387.1 cation transporter dimerization domain-containing protein -
HRG83_RS29460 5000744..5000848 - 105 Protein_4862 hypothetical protein -
HRG83_RS24740 (HRG83_24735) 5000873..5001112 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HRG83_RS24745 (HRG83_24740) 5001112..5001399 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HRG83_RS24750 (HRG83_24745) 5001471..5001629 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
HRG83_RS24755 (HRG83_24750) 5002232..5002954 + 723 WP_228293780.1 Hok/Gef family protein -
HRG83_RS24765 (HRG83_24760) 5004656..5005033 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4986527..5038338 51811
- inside IScluster/Tn - - 4995935..5004128 8193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T197849 WP_000323025.1 NZ_CP072463:5001112-5001399 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T197849 NZ_CP096776:c4273151-4271838 [Proteus mirabilis]
ATGGCGAGTCTTGATGTATATATGAATGAATACTATGTGGGCATCTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATACTGAAGATTGGGTTAATCAACCAGGAAGTCGTCCCATCTCTCTTTCGATGCCATTACAGCTTCAAGAATATCAAG
GAGACTGTGTCTATAATTTCTTTGATAATCTGTTACCTGATAATACTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCGAACTCAAATCAACCCTTTGACCTATTAGCGGCTATCGGTGCAGATACTGTTGGTGCATTAAGGTTACTTCCTAGTGG
CAGTACGCCTGCGGATATCAAAAAAATAGAATATCAAAAACTCAATGATACTGAGCTTGAAAAAATTCTACTAGGATATA
AATCCAATACTCCACTCGGTATGCTTAAAGAATATACTGATTTCAGAATTTCCATCGCTGGAGCACAAGAAAAAACGGCA
TTACTTTTAAAAGATAATCATTGGTGTGTGCCACATAATAACACACCAACTACCCATATTATTAAACTGCCAATTGGTGT
TATTGAAAGTCATTCTTATACAATGGATTTATCCAATAGTGTAGAAAATGAGTATCTTTGTACCTTAATTGCCAAAGAAT
TTGATCTTCCCGTCCCCCACTGCTCTATTATTAAAACAAAAAATGTTAAAGCGCTTGCTGTAGAACGTTTTGATAGAAAA
TATTCATCTGATAAATCGTGGATAATACGTTTACCTCAAGAAGATTTTTGCCAAATTTTAAATATACCTTCTGCGCTAAA
ATATGAAAATGAAGGAGGGCCTGGCATTATCGATATTATGAAATATTTACTTGGTTCATCAAATGCCGATCAAGATCGTT
TTCATTTTATGAAGGCACAAGTGCTATTTTGGCTTTTGGCTGCAACGGATGGACACGCAAAGAATTTTTCTGTTTTTATC
AACCAAGAAGGACGTTTCCATCTCACTCCCCTTTATGACATTATGTCCATCTATCCCAATTTCGGAGGACGAGGACTTAA
TCCACGAGATGCAAAACTTGCTATGGGATTAAAAGCAAGCCGAGGCAAAAAATACGCAATAGAACAAATTTTTCCTCGTC
ATTTCTACCAAACAGCTGAAGCTGTCGGTTTCGATCCTTCATTAATGAGCGATATTCTTCGTTATTTTTATCAAGAAATG
GACGATGTCATACAACGCGTAAAACAGCAATTACCCGAGGATTTCCCTTCTGAGATTAGCAATACAATATTAGATGGAAT
GAAAGCACGCGCTGTTAGATTAGCTAACATTTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT197849 WP_000534858.1 NZ_CP072463:5000873-5001112 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT197849 NZ_CP096776:c4273409-4273152 [Proteus mirabilis]
ATGATCATAATTAATGCCTATCAACTCAGTGTGCATCTAAAAGATGTCCGATTAACAAAAAAACTGTCTCAAAGTAAAGT
CGCCCAAAAAGTAGGGATCCGACAAGATACCGTGTCCAATTTTGAACTTAATCCTAATTCCACCAAACTAGAAACCTTTT
TCAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References