Detailed information of TA system TA197832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 179412..180055
• Replicon: plasmid pK-1L-1
• Accession: NZ_CP072461
• Organism: Klebsiella pneumoniae strain K-1L

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: J7V83_RS27530
• Protein ID: WP_001044768.1
• Coordinates: 179412..179828 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: J7V83_RS27535
• Protein ID: WP_001261287.1
• Coordinates: 179825..180055 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V83_RS27515 (J7V83_27365)	175911..176501	-	591	WP_000194575.1	hypothetical protein	-
J7V83_RS27520 (J7V83_27370)	176501..176758	-	258	WP_000343085.1	hypothetical protein	-
J7V83_RS27525 (J7V83_27375)	177112..179250	+	2139	WP_000350638.1	AAA family ATPase	-
J7V83_RS27530 (J7V83_27380)	179412..179828	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7V83_RS27535 (J7V83_27385)	179825..180055	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7V83_RS27540 (J7V83_27390)	180351..180623	+	273	WP_061602529.1	hypothetical protein	-
J7V83_RS27545 (J7V83_27395)	180670..181374	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
J7V83_RS27550 (J7V83_27400)	181421..181855	-	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
J7V83_RS27555 (J7V83_27405)	181927..182277	+	351	WP_001294663.1	mercuric transport protein MerT	-
J7V83_RS27560 (J7V83_27410)	182291..182566	+	276	WP_000732292.1	mercury resistance system periplasmic binding protein MerP	-
J7V83_RS27565 (J7V83_27415)	182602..183024	+	423	WP_001340589.1	organomercurial transporter MerC	-
J7V83_RS27570 (J7V83_27420)	183076..184770	+	1695	WP_000105636.1	mercury(II) reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	lnu(G) / tet(X4) / floR / qnrS1 / tet(A) / aph(3')-Ia / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / dfrA14 / blaOXA-10 / ARR-3 / blaTEM-1B	-	1..301103	301103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T197832 WP_001044768.1 NZ_CP072461:c179828-179412 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T197832 NZ_CP096761:c7973-7686 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGATATACTAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT197832 WP_001261287.1 NZ_CP072461:c180055-179825 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT197832 NZ_CP096761:c7699-7385 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4