Detailed information of TA system TA197822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3463927..3464737
• Replicon: chromosome
• Accession: NZ_CP072460
• Organism: Klebsiella pneumoniae strain K-1L

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A483G813
• Locus tag: J7V83_RS17155
• Protein ID: WP_023279404.1
• Coordinates: 3463927..3464460 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: J7V83_RS17160
• Protein ID: WP_186593478.1
• Coordinates: 3464471..3464737 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V83_RS17150 (3462758)	3462758..3463879	+	1122	WP_002887282.1	cupin domain-containing protein	-
J7V83_RS17155 (3463927)	3463927..3464460	-	534	WP_023279404.1	type II toxin-antitoxin system toxin KacT	Toxin
J7V83_RS17160 (3464471)	3464471..3464737	-	267	WP_186593478.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
J7V83_RS17165 (3464840)	3464840..3466273	-	1434	WP_004192214.1	Cu(+)/Ag(+) sensor histidine kinase	-
J7V83_RS17170 (3466263)	3466263..3466946	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
J7V83_RS17175 (3467118)	3467118..3468503	+	1386	WP_004192218.1	efflux transporter outer membrane subunit	-
J7V83_RS17180 (3468521)	3468521..3468865	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19809.71 Da
• Isoelectric Point: 6.2369

>T197822 WP_023279404.1 NZ_CP072460:c3464460-3463927 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T197822 NZ_CP096753:3346100-3346279 [Acinetobacter baumannii]
ATGAGCTTCAACGAGTTTAAACGATGGCTAATTGCACAGGGAGTTATCTTTGTGCGTAAAGGTAAGGGATCACATATGAT
TATTGAATTTAACGGTAAGAAGACTGTTTTCCCTAACCACGGGAAAAAAGAAATTCCTGAGGGCACTCGTTTAAAAATCA
AAAAGGATTTGGGGCTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9897.33 Da
• Isoelectric Point: 8.8077

>AT197822 WP_186593478.1 NZ_CP072460:c3464737-3464471 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTG

Nucleotide

• Length: 267 bp

>AT197822 NZ_CP096753:3346369-3346782 [Acinetobacter baumannii]
ATGAAATATTACGTGAGTATAGTACAGGAGGAAGGAGCTTATATAGTTAGCTCTCGGGACCTTCCTCTGTTAAATAGCGT
AGGCTATACATTAGAAGAAGCTTTATCTGAAGCTTTGGATGGTATAGAAACTACTTTCGAAATTTATATGGATGAGCGTA
AGGCTATTCCTTTACCATCAAAAGGAAAGAAAGATGAATATGAAATTCATCTTCCAGTTCGAGTTGCTGCTAAGGTACGT
TTATATAATGAGATGATCTCTCAAGACGTTACTAAGGCTGAATTAGCTCGTCGCTTGGGGTGGTTACAGAAGCAAGCCGA
CCGTTTGCTTTCATTAAAGCATTCAACAAAATTAGAGTCTATCGAAAGTGCATTTCATGCATTAGGTAAAGATCTCGATA
TTGTTGTTGCATAA

Structures

Toxin

• AlphaFold DB: A0A483G813