Detailed information of TA system TA197810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 68468..69111
• Replicon: plasmid pb1-2L-2
• Accession: NZ_CP072458
• Organism: Klebsiella pneumoniae strain b1-2L

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: J7V71_RS27970
• Protein ID: WP_001044768.1
• Coordinates: 68468..68884 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: J7V71_RS27975
• Protein ID: WP_001261287.1
• Coordinates: 68881..69111 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V71_RS27955 (J7V71_27955)	64967..65557	-	591	WP_000194575.1	hypothetical protein	-
J7V71_RS27960 (J7V71_27960)	65557..65814	-	258	WP_000343085.1	hypothetical protein	-
J7V71_RS27965 (J7V71_27965)	66168..68306	+	2139	WP_000350638.1	AAA family ATPase	-
J7V71_RS27970 (J7V71_27970)	68468..68884	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7V71_RS27975 (J7V71_27975)	68881..69111	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7V71_RS27980 (J7V71_27980)	69407..69679	+	273	WP_061602529.1	hypothetical protein	-
J7V71_RS27985 (J7V71_27985)	69726..70430	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
J7V71_RS27990 (J7V71_27990)	70477..70911	-	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
J7V71_RS27995 (J7V71_27995)	70983..71333	+	351	WP_001294663.1	mercuric transport protein MerT	-
J7V71_RS28000 (J7V71_28000)	71347..71622	+	276	WP_000732292.1	mercury resistance system periplasmic binding protein MerP	-
J7V71_RS28005 (J7V71_28005)	71658..72080	+	423	WP_001340589.1	organomercurial transporter MerC	-
J7V71_RS28010 (J7V71_28010)	72132..73826	+	1695	WP_000105636.1	mercury(II) reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / aph(3')-Ia / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / floR / dfrA14 / blaOXA-10 / ARR-3 / qnrS1	-	1..111105	111105
	-	flank	IS/Tn	-	-	69726..70430	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T197810 WP_001044768.1 NZ_CP072458:c68884-68468 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T197810 NZ_CP096740:452018-452407 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT197810 WP_001261287.1 NZ_CP072458:c69111-68881 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT197810 NZ_CP096740:451754-452011 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4