Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5341391..5342016 Replicon chromosome
Accession NZ_CP072456
Organism Klebsiella pneumoniae strain b1-2L

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag J7V71_RS26310 Protein ID WP_004187928.1
Coordinates 5341391..5341774 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J7V71_RS26315 Protein ID WP_004150355.1
Coordinates 5341774..5342016 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V71_RS26295 (5338757) 5338757..5339659 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J7V71_RS26300 (5339656) 5339656..5340291 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J7V71_RS26305 (5340288) 5340288..5341217 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J7V71_RS26310 (5341391) 5341391..5341774 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
J7V71_RS26315 (5341774) 5341774..5342016 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J7V71_RS26320 (5342210) 5342210..5343127 + 918 WP_004187929.1 alpha/beta hydrolase -
J7V71_RS26325 (5343141) 5343141..5344082 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J7V71_RS26330 (5344127) 5344127..5344564 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J7V71_RS26335 (5344561) 5344561..5345421 - 861 WP_004146232.1 virulence factor BrkB family protein -
J7V71_RS26340 (5345415) 5345415..5346014 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T197809 WP_004187928.1 NZ_CP072456:c5341774-5341391 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T197809 NZ_CP096739:c13055-12696 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT197809 WP_004150355.1 NZ_CP072456:c5342016-5341774 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT197809 NZ_CP096739:c12696-12397 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References