Detailed information of TA system TA197805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4725395..4726205
• Replicon: chromosome
• Accession: NZ_CP072456
• Organism: Klebsiella pneumoniae strain b1-2L

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A483G813
• Locus tag: J7V71_RS23375
• Protein ID: WP_023279404.1
• Coordinates: 4725395..4725928 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: J7V71_RS23380
• Protein ID: WP_186593478.1
• Coordinates: 4725939..4726205 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V71_RS23370 (4724226)	4724226..4725347	+	1122	WP_002887282.1	cupin domain-containing protein	-
J7V71_RS23375 (4725395)	4725395..4725928	-	534	WP_023279404.1	type II toxin-antitoxin system toxin KacT	Toxin
J7V71_RS23380 (4725939)	4725939..4726205	-	267	WP_186593478.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
J7V71_RS23385 (4726308)	4726308..4727741	-	1434	WP_004192214.1	Cu(+)/Ag(+) sensor histidine kinase	-
J7V71_RS23390 (4727731)	4727731..4728414	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
J7V71_RS23395 (4728586)	4728586..4729971	+	1386	WP_004192218.1	efflux transporter outer membrane subunit	-
J7V71_RS23400 (4729989)	4729989..4730333	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19809.71 Da
• Isoelectric Point: 6.2369

>T197805 WP_023279404.1 NZ_CP072456:c4725928-4725395 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T197805 NZ_CP096731:c2231810-2231628 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9897.33 Da
• Isoelectric Point: 8.8077

>AT197805 WP_186593478.1 NZ_CP072456:c4726205-4725939 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTG

Nucleotide

• Length: 267 bp

>AT197805 NZ_CP096731:c2231535-2231131 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: A0A483G813