Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4115274..4115893 Replicon chromosome
Accession NZ_CP072456
Organism Klebsiella pneumoniae strain b1-2L

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J7V71_RS20425 Protein ID WP_002892050.1
Coordinates 4115675..4115893 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag J7V71_RS20420 Protein ID WP_002892066.1
Coordinates 4115274..4115648 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V71_RS20410 (4110426) 4110426..4111619 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J7V71_RS20415 (4111642) 4111642..4114788 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
J7V71_RS20420 (4115274) 4115274..4115648 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
J7V71_RS20425 (4115675) 4115675..4115893 + 219 WP_002892050.1 HHA domain-containing protein Toxin
J7V71_RS20430 (4116052) 4116052..4116618 + 567 WP_002892030.1 maltose O-acetyltransferase -
J7V71_RS20435 (4116590) 4116590..4116730 - 141 WP_004147370.1 hypothetical protein -
J7V71_RS20440 (4116751) 4116751..4117221 + 471 WP_002892026.1 YlaC family protein -
J7V71_RS20445 (4117196) 4117196..4118647 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
J7V71_RS20450 (4118748) 4118748..4119446 + 699 WP_002892021.1 GNAT family protein -
J7V71_RS20455 (4119443) 4119443..4119583 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
J7V71_RS20460 (4119583) 4119583..4119846 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197804 WP_002892050.1 NZ_CP072456:4115675-4115893 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T197804 NZ_CP096731:1017065-1017247 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT197804 WP_002892066.1 NZ_CP072456:4115274-4115648 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT197804 NZ_CP096731:1017312-1017716 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References