Detailed information of TA system TA197783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1290345..1290989
• Replicon: chromosome
• Accession: NZ_CP072455
• Organism: Xenorhabdus budapestensis strain C-7-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A077QJ13
• Locus tag: HGO23_RS05920
• Protein ID: WP_038186411.1
• Coordinates: 1290345..1290518 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HGO23_RS05925
• Protein ID: WP_209028274.1
• Coordinates: 1290573..1290989 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO23_RS05890 (HGO23_05890)	1285433..1286809	+	1377	WP_209028269.1	TIGR03752 family integrating conjugative element protein	-
HGO23_RS05895 (HGO23_05895)	1286846..1287202	+	357	WP_209028270.1	hypothetical protein	-
HGO23_RS05900 (HGO23_05900)	1287190..1287591	+	402	WP_209028271.1	TIGR03751 family conjugal transfer lipoprotein	-
HGO23_RS05905 (HGO23_05905)	1287591..1287920	+	330	Protein_1170	TraC family protein	-
HGO23_RS05915 (HGO23_05915)	1289169..1290212	+	1044	Protein_1172	hypothetical protein	-
HGO23_RS05920 (HGO23_05920)	1290345..1290518	+	174	WP_038186411.1	type II toxin-antitoxin system HicA family toxin	Toxin
HGO23_RS05925 (HGO23_05925)	1290573..1290989	+	417	WP_209028274.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HGO23_RS05930 (HGO23_05930)	1291644..1292150	-	507	WP_209028275.1	zinc-ribbon domain-containing protein	-
HGO23_RS05940 (HGO23_05940)	1292721..1293203	-	483	WP_099137227.1	SsrA-binding protein SmpB	-
HGO23_RS05945 (HGO23_05945)	1293360..1293794	+	435	WP_099137228.1	type II toxin-antitoxin system RatA family toxin	-
HGO23_RS05950 (HGO23_05950)	1293787..1294080	+	294	WP_099137229.1	RnfH family protein	-
HGO23_RS05955 (HGO23_05955)	1294141..1294485	-	345	WP_209028276.1	outer membrane protein assembly factor BamE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1251617..1334076	82459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (90-130)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6652.78 Da
• Isoelectric Point: 11.1572

>T197783 WP_038186411.1 NZ_CP072455:1290345-1290518 [Xenorhabdus budapestensis]
VKQSEFRRWLEAQGVEVSNGTNHLKLKYQGKRSIMPRHPSQEIKEPLRKAIIKQLGL

Nucleotide

• Length: 174 bp

>T197783 NZ_CP096713:4851-5207 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15344.37 Da
• Isoelectric Point: 4.1716

>AT197783 WP_209028274.1 NZ_CP072455:1290573-1290989 [Xenorhabdus budapestensis]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGDTREEALEMALDALITSFEFYFEDNEKIPLPSSVGQDDDYVDVPLSVASKV
LMLNAFVDSKLTQTELASRMGVKKQEVTRIFDLRHSTKIDTVGKVASAIGHQLTLSIE

Nucleotide

• Length: 417 bp

>AT197783 NZ_CP096713:5200-5502 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A077QJ13