Detailed information of TA system TA197780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 591190..592541
• Replicon: chromosome
• Accession: NZ_CP072455
• Organism: Xenorhabdus budapestensis strain C-7-2

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: HGO23_RS02555
• Protein ID: WP_209027867.1
• Coordinates: 591597..592541 (+)
• Length: 315 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A077QN51
• Locus tag: HGO23_RS02550
• Protein ID: WP_038189491.1
• Coordinates: 591190..591594 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO23_RS02515 (HGO23_02515)	586200..586883	-	684	WP_209027864.1	N-6 DNA methylase	-
HGO23_RS02520 (HGO23_02520)	586986..587909	-	924	WP_209027865.1	DUF1281 domain-containing protein	-
HGO23_RS02525 (HGO23_02525)	588001..588297	-	297	WP_010846350.1	TA system toxin CbtA family protein	-
HGO23_RS02530 (HGO23_02530)	588313..588843	-	531	WP_010846351.1	hypothetical protein	-
HGO23_RS02535 (HGO23_02535)	588965..589426	-	462	WP_010846352.1	antirestriction protein	-
HGO23_RS19515 (HGO23_02540)	589416..589763	-	348	WP_280529535.1	antirestriction protein	-
HGO23_RS19520	590850..591005	+	156	WP_038189488.1	hypothetical protein	-
HGO23_RS02550 (HGO23_02550)	591190..591594	+	405	WP_038189491.1	DUF6088 family protein	Antitoxin
HGO23_RS02555 (HGO23_02555)	591597..592541	+	945	WP_209027867.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HGO23_RS02560 (HGO23_02560)	592550..595144	+	2595	WP_038189494.1	type I restriction-modification system subunit M	-
HGO23_RS02565 (HGO23_02565)	595137..596411	+	1275	WP_209027868.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	583529..682037	98508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (32-301)

Antitoxin

• Protein of unknown function DUF6088 (6-129)

Sequences

Toxin

Protein

• Length: 315 a.a.
• Molecular weight: 36421.01 Da
• Isoelectric Point: 6.3098

>T197780 WP_209027867.1 NZ_CP072455:591597-592541 [Xenorhabdus budapestensis]
MKKLKDQFLEVSDALELGNPAIIEKDYWVVALLAELAKLAFDTHQMVFSGGTALAKSNVKILRMSEDVDIKLIPTDAFNS
FGRDKRKATRKALVQQIEESLIHQSSHFSIKEKKARDEYRYVEYELKYPQQFRQAPCLRPIIKLELMESIPLLQIEQRSI
QSLVAELYHQPHEVNAFSCISVQSTLVEKIISMLRRTMLVKRDSERKDDATLIRHIYDVHCIDVDQKLDFETLMPLFQTV
LQEDIKRFGNQHAEFVANPVQELQLGLQELESNPLFCKRFTDFVTPMVFNTETPDFDTCFASFKRIAQSLMNNN

Nucleotide

• Length: 945 bp

>T197780 NZ_CP072455:591597-592541 [Xenorhabdus budapestensis]
ATGAAAAAATTAAAAGATCAATTCTTAGAGGTTTCCGATGCCTTAGAGCTCGGAAACCCAGCCATTATTGAAAAAGATTA
CTGGGTAGTGGCTTTGCTTGCTGAGTTAGCAAAGCTTGCTTTTGATACGCATCAAATGGTTTTTTCTGGCGGGACGGCAC
TGGCTAAATCCAATGTAAAAATCCTCAGGATGTCGGAAGATGTCGACATCAAACTCATCCCAACGGATGCATTTAACTCA
TTCGGTCGTGACAAGCGCAAGGCGACACGTAAAGCTCTGGTGCAACAAATCGAAGAATCTCTAATTCACCAATCAAGCCA
TTTTTCTATTAAAGAAAAAAAAGCCCGTGATGAATACCGCTACGTCGAATATGAACTGAAATACCCACAGCAATTTAGAC
AAGCACCTTGCTTGCGCCCAATCATTAAACTGGAACTCATGGAGAGCATACCTCTGCTTCAAATAGAACAGCGTAGCATT
CAATCACTGGTTGCTGAGCTGTATCATCAGCCTCACGAAGTCAACGCATTTTCTTGTATCTCCGTTCAATCCACTTTGGT
GGAGAAAATCATTTCTATGCTGCGCCGTACCATGCTAGTCAAACGAGATTCTGAGCGCAAAGATGATGCTACGTTGATAC
GCCATATCTACGATGTACATTGTATTGACGTAGACCAGAAACTTGATTTTGAAACTCTTATGCCCCTTTTCCAAACCGTG
TTACAAGAAGACATTAAGCGATTTGGTAATCAGCATGCGGAGTTTGTTGCTAACCCTGTTCAAGAATTACAGCTAGGTTT
GCAAGAGCTAGAGTCTAACCCGCTGTTTTGTAAAAGGTTTACAGACTTTGTCACCCCAATGGTGTTTAATACAGAAACAC
CCGATTTCGATACCTGCTTTGCCTCATTTAAGCGCATTGCTCAGTCATTGATGAATAATAATTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15169.40 Da
• Isoelectric Point: 10.6956

>AT197780 WP_038189491.1 NZ_CP072455:591190-591594 [Xenorhabdus budapestensis]
MTAADRINQKLKRSRRYVFERKDFEDIASYDQIGRALNQLIKQGELMKIGYGLYTKARKNSLTGRLMPTNPGGTDAILRE
ILKMKGVDFEVDSLSLQSLSGNSTQIPSSVQYSWNPKQFNRKLVVGNRVLNSPS

Nucleotide

• Length: 405 bp

>AT197780 NZ_CP072455:591190-591594 [Xenorhabdus budapestensis]
ATGACAGCAGCAGATCGAATCAATCAAAAGTTGAAACGTTCGAGACGTTACGTCTTTGAACGCAAAGATTTTGAAGACAT
TGCCAGTTATGACCAGATCGGCCGTGCGCTTAATCAATTGATCAAGCAAGGTGAACTGATGAAAATCGGATATGGCCTGT
ATACTAAAGCACGTAAGAACAGCCTCACAGGTCGCCTAATGCCAACGAATCCTGGTGGCACCGATGCGATTTTACGTGAA
ATTCTTAAGATGAAAGGCGTAGACTTCGAAGTAGATAGTCTATCGTTGCAAAGTCTTTCCGGTAATAGCACACAAATCCC
TTCATCTGTGCAGTATTCGTGGAATCCTAAGCAGTTTAATCGCAAGCTGGTTGTTGGTAATCGCGTACTTAACTCACCAA
GTTAA

Structures

Antitoxin

• AlphaFold DB: A0A077QN51