Detailed information of TA system TA197777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 3572084..3572667
• Replicon: chromosome
• Accession: NZ_CP072453
• Organism: Providencia sp. R33

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: J6836_RS16790
• Protein ID: WP_219245056.1
• Coordinates: 3572365..3572667 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J6836_RS16785
• Protein ID: WP_219245055.1
• Coordinates: 3572084..3572368 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6836_RS16760 (J6836_16765)	3567269..3568246	-	978	WP_219245050.1	6-phosphofructokinase	-
J6836_RS16765 (J6836_16770)	3568642..3569586	-	945	WP_219245051.1	phosphatidate cytidylyltransferase	-
J6836_RS16770 (J6836_16775)	3569583..3570224	-	642	WP_219245052.1	lysophospholipid acyltransferase family protein	-
J6836_RS16775 (J6836_16780)	3570443..3571195	-	753	WP_219245053.1	M48 family metalloprotease	-
J6836_RS16780 (J6836_16785)	3571423..3572004	-	582	WP_219245054.1	HutD family protein	-
J6836_RS16785 (J6836_16790)	3572084..3572368	-	285	WP_219245055.1	putative addiction module antidote protein	Antitoxin
J6836_RS16790 (J6836_16795)	3572365..3572667	-	303	WP_219245056.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6836_RS16795 (J6836_16800)	3572925..3573251	-	327	WP_255586247.1	hypothetical protein	-
J6836_RS16800 (J6836_16805)	3573357..3573548	-	192	WP_255586422.1	ogr/Delta-like zinc finger family protein	-
J6836_RS16805 (J6836_16810)	3573651..3574757	-	1107	WP_219245058.1	phage late control D family protein	-
J6836_RS16810 (J6836_16815)	3574916..3576079	+	1164	WP_219245059.1	phage tail sheath subtilisin-like domain-containing protein	-
J6836_RS16815 (J6836_16820)	3576092..3576604	+	513	WP_219245060.1	phage major tail tube protein	-
J6836_RS16820 (J6836_16825)	3576665..3576967	+	303	WP_123381962.1	phage tail assembly protein	-
J6836_RS22800	3577051..3577116	+	66	WP_230083332.1	GpE family phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3572084..3603054	30970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11507.37 Da
• Isoelectric Point: 10.3594

>T197777 WP_219245056.1 NZ_CP072453:c3572667-3572365 [Providencia sp. R33]
MITVLTTDLFDNWIRDLKDLRAKTKIQVRIRRLKNGNFGDIEPIGDGFSELRIHEGQGYRVYLKQKNNVMVILLFGGSKG
TQQKDIIKAKQVFSQIEGEL

Nucleotide

• Length: 303 bp

>T197777 NZ_CP096704:451052-451441 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10422.05 Da
• Isoelectric Point: 4.8090

>AT197777 WP_219245055.1 NZ_CP072453:c3572368-3572084 [Providencia sp. R33]
MTIKLKEYDIAEHLNSEEEIQLYLNEILEEGDPALLLSALGDIAKARNMSQLAKEVGMSREGLYKALSGQGNPTFNTIIK
IVNSLGLKLQFKSI

Nucleotide

• Length: 285 bp

>AT197777 NZ_CP096704:450788-451045 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA